Y染色体性别决定区相关高速泳动族框因子9和周期素依赖性激酶4在结直肠息肉、结直肠癌中的表达及意义  

Expression and significance of SOX9 and CDK4 in colorectal polyps and colorectal cancer

作　　者：赵玉红 张磊[1] 古彩喆 张东姣 杨卫振[2] ZHAO Yuhong;ZHANG Lei;GU Caizhe;ZHANG Dongjiao;YANG Weizhen(Department of Gastroenterology,No.82 Military Hospital of PLA,Baoding,Hebei 071000,China;Department of General Surgery,Baoding First Central Hospital,Baoding,Hebei 071000,China)

机构地区：[1]中国人民解放军第八十二集团军医院消化内科,河北保定071000 [2]保定市第一中心医院普外二科,河北保定071000

出　　处：《安徽医药》2023年第6期1170-1174,共5页Anhui Medical and Pharmaceutical Journal

基　　金：保定市科技计划项目(18ZF181)。

摘　　要：目的 探究Y染色体性别决定区相关高速泳动族框因子9(SOX9)和周期素依赖性激酶4(CDK4)在结直肠息肉、结直肠癌中的表达及意义。方法 选取中国人民解放军第八十二集团军医院2015年1月至2017年6月收治的结直肠癌病人病理组织标本(结直肠癌组)94例及癌旁正常结直肠组织(正常结直肠组)39例,另取同时期结直肠息肉病人结直肠息肉组织(结直肠息肉组)51例。采用免疫组织化学SP染色法检测SOX9、CDK4的表达情况,分析SOX9、CDK4表达与结直肠癌病人临床病理参数之间的关系;对结直肠癌病人进行术后复查随访,分析结直肠癌病人生存情况。结果 SOX9在正常结直肠组织、结直肠息肉组织和结直肠癌组织中的阳性表达率分别为10.26%(4/39)、27.45%(14/51)、76.59%(72/94),差异有统计学意义(P<0.05);CDK4在正常结直肠组织、结直肠息肉组织和结直肠癌组织中的阳性表达率分别为7.69%(3/39)、21.57%(11/51)、54.84%(51/94),差异有统计学意义(P<0.05);正常结直肠组织中SOX9、CDK4蛋白表达水平低于结直肠息肉组织、结直肠癌组织(P<0.05),结直肠息肉组织中SOX9、CDK4蛋白表达水平低于结直肠癌组织(P<0.05)。结直肠癌病人病理组织SOX9、CDK4表达与肿瘤分化程度、临床分期、是否合并淋巴结转移有关(P<0.05);结直肠癌组织SOX9与CDK4表达呈正相关(r=0.58,P<0.001),由TCGA数据库检索结直肠癌组织SOX9、CDK4基因表达相关性显示,二者之间亦呈正相关(r=0.40,P<0.001);SOX9高表达、低表达病人术后3年累积生存率分别为44.6%、72.4%,两组比较Log-rank检验差异有统计学意义(χ^(2)=8.31,P=0.004);CDK4高表达、低表达病人术后3年累积生存率分别为25.6%、76.5%,两组比较Log-rank检验差异有统计学意义(χ^(2)=36.36,P<0.001)。结论 SOX9和CDK4在结直肠癌组织、结直肠息肉组织、正常结直肠组织中的阳性表达率逐渐降低,与结直肠癌肿瘤分化程度、临床分期�Objective To investigate the expression and significance of sex-determining region Y(SRY)-box 9 protein(SOX9)and cyclin-dependent kinase 4(CDK4)in colorectal polyps and colorectal cancer.Methods Ninety-four cases of pathological tissue specimens of colorectal cancer patients and 39 cases of adjacent normal colorectal tissue treated in The 82nd Group Military Hospital of the People's Liberation Army from January 2015 to June 2017 were selected,and another 51 cases of colorectal polyps were taken from patients with colorectal polyps at the same time.The expression of SOX9 and CDK4 was detected by immunohistochemical SP staining,the relationship between the expression of SOX9 and CDK4 and clinicopathological parameters was analyzed;the patients with colorectal cancer were followed up after operation to analyze the survival of patients with colorectal cancer.Results The positive expression rate of SOX9 in normal colorectal tissues,colorectal polyps and colorectal cancer was 10.26%(4/39),27.45%(14/51)and 76.59%(72/94),respectively,and the difference was statistically significant(P<0.05).The positive expression rate of CDK4 in normal colorectal tissues,colorectal polyps and colorectal cancer was 7.69%(3/39),21.57%(11/51)and 54.84%(51/94),respectively,and the difference was statistically significant(P<0.05).The expression levels of SOX9 and CDK4 protein in normal colorectal tissues were lower than those in colorectal polyps and colorectal cancer,and the difference was statistically significant(P<0.05),and the expression levels of SOX9 and CDK4 protein in colorectal polyps were lower than those in colorectal cancer,and the difference was statistically significant(P<0.05).The expression of SOX9 and CDK4 in colorectal cancer was correlated with tumor differentiation,clinical stage and lymph node metastasis(P<0.05).There was a positive correlation between SOX9 and CDK4 expression in colorectal cancer(r=0.58,P<0.001),and there was a positive correlation between SOX9 and CDK4 gene expression in colorectal cancer retrieved fro

关 键 词：结直肠肿瘤 Y染色体性别决定区相关高速泳动族框因子9 周期素依赖性激酶4 基因表达 细胞分化 淋巴转移 

分 类 号：R735.34[医药卫生—肿瘤]