微RNA-125a-5p和维生素D受体微RNA在乙型肝炎病毒相关性肝癌中的表达及与预后的关系  

Expression of miR-125a-5p and vitamin D receptor mRNA in hepatitis B virus-related liver cancer and their relationship with prognosis

作　　者：李玉苓 邹东花 高健[3] LI Yuling;ZOU Donghua;GAO Jian(Department of Hepatology with Integrated Chinese and Western Medicine,Cangzhou,Hebei 061000,Chin;Laboratory,Cangzhou Third Hospital,Cangzhou,Hebei 061000,China;The Department of Hepatobiliary Surgery,The Third Hospital of Hebei Medical University,Shijiazhuang,Hebei 050000,China)

机构地区：[1]沧州市第三医院中西医结合肝病科,河北沧州061000 [2]沧州市第三医院检验科,河北沧州061000 [3]河北医科大学第三医院肝胆外科,河北石家庄050000

出　　处：《安徽医药》2023年第6期1178-1182,I0008,共6页Anhui Medical and Pharmaceutical Journal

基　　金：沧州市科学技术局资助项目(172302049)。

摘　　要：目的 探讨微RNA-125a-5p(miR-125a-5p)和维生素D受体(VDR)在乙型肝炎病毒(HBV)相关肝细胞癌(肝癌)病人组织中的表达情况及临床意义。方法 选取2014年2月至2016年5月在沧州市第三医院接受手术治疗的HBV相关肝癌病人65例,术中取病人肝癌组织及癌旁组织,比较miR-125a-5p、VDR mRNA在不同肝组织中的表达差异,分析miR-125a-5p、VDR mRNA水平与肝癌病人临床病理特征的关系,采用Pearson法分析miR-125a-5p、VDR mRNA的相关性。随访5年,Kaplan-Meier法分析miR-125a-5p、VDR mRNA水平对肝癌病人生存率的影响。结果 肝癌组织miR-125a-5p水平0.69±0.16低于癌旁组织0.97±0.11(P<0.05),VDR mRNA水平1.73±0.45高于癌旁组织1.03±0.12(P<0.05)。miR-125a-5p、VDR mRNA水平与HBV相关肝癌病人门静脉侵袭、淋巴结转移、分化程度及TNM分期关系密切(P<0.05)。肝癌病人肝癌组织中miR-125a-5p与VDR mRNA呈负相关(r=-0.44,P<0.001)。miR-125a-5p高表达组5年生存率为65.63%,高于miR-125a-5p低表达组的39.39%(P<0.05);VDR mRNA高表达组5年生存率为37.50%,低于VDR mRNA低表达组的66.67%(P<0.05)。miR-125a-5p高表达+VDR mRNA低表达病人、miR-125a-5p低表达+VDR mRNA高表达病人、miR-125a-5p高表达+VDR mRNA高表达病人、miR-125a-5p低表达+VDR mRNA低表达病人5年生存率分别为69.57%、23.81%、63.64%、60.00%,四组比较差异有统计学意义(P<0.05)。结论 HBV相关肝癌病人肝癌组织中miR-125a-5p表达降低,VDR mRNA水平升高,二者与门静脉侵袭、淋巴结转移、分化程度及TNM分期有关,miR-125a-5p高表达及VDR mRNA低表达病人5年生存率较高,对于预后评估有一定价值。Objective To investigate the expression and clinical significance of microRNA-125a-5p(miR-125a-5p)and vitamin D receptor(VDR)in the tissues of hepatitis B virus(HBV)-related hepatocellular carcinoma(liver cancer)patients.Methods From February 2014 to May 2016,Sixty-five HBV-related liver cancer patients who underwent surgical treatment in Cangzhou Third Hospital were selected.Liver cancer tissues and para-cancerous tissues were collected from the patients during the operation,and the survival status of the patients was followed up for 5 years.The expression differences of miR-125a-5p and VDR mRNA in different liver tissues were compared,the relationship between miR-125a-5p,VDR mRNA levels and clinicopathological characteristics of liver cancer patients was analyzed,the correlation between miR-125a-5p and VDR mRNA was analyzed with Pearson method,the effects of miR-125a-5p and VDR mRNA levels on the survival rate of liver cancer patients were analyzed with Kaplan-Meier method.Results The level of miR-125a-5p 0.69±0.16 in liver cancer tissues was lower than that of adjacent tissues 0.97±0.11(P<0.05),and the level of VDR mRNA 1.73±0.45 was higher than that of adjacent tissues 1.03±0.12(P<0.05).The levels of miR-125a-5p and VDR mRNA were closely related to portal vein invasion,lymph node metastasis,degree of differentiation and TNM stage in patients with HBV-related liver cancer(P<0.05).There was a negative correlation between miR-125a-5p and VDR mRNA in liver cancer tissues of patients with liver cancer(r=−0.44,P<0.001).The 5-year survival rate of miR-125a-5p high expression group was 65.63%,which was higher than 39.39%of miR-125a-5p low expression group(P<0.05);The 5-year survival rate of the VDR mRNA high expression group was 37.50%,which was lower than the 66.67%of the VDR mRNA low expression group(P<0.05).The 5-year survival rates of the patients with high expression of miR-125a-5p+low expression of VDR mRNA,patients with low expression of miR-125a-5p+high expression of VDR mRNA,patients with high expressi

关 键 词：肝肿瘤 微RNA-125a-5p 维生素D受体 乙型肝炎病毒相关性肝癌 预后 

分 类 号：R735.7[医药卫生—肿瘤] R512.62[医药卫生—临床医学]