BWEL-SPF鸡基因组内源禽白血病病毒基因插入位点识别和验证  

Identification and validation of endogenous Avian leukosis virus insertion sites in BWEL-SPF chicken genome

作　　者：王梓一 袁一铭 连玲[1] WANG Ziyi;YUAN Yiming;LIAN Ling(College of Animal Science and Technology,China Agricultural University,Beijing 100193,China)

机构地区：[1]中国农业大学动物科学技术学院,北京100193

出　　处：《黑龙江畜牧兽医》2023年第7期15-19,25,共6页Heilongjiang Animal Science And veterinary Medicine

基　　金：国家自然科学基金项目(U1901206);国家重点研发计划项目(2021YFD1300600);家养动物种质资源库项目(CARS-40)。

摘　　要：为了探究生物信息学软件识别禽白血病病毒E(Avian leukosis virus subgroup E, ALVE)基因插入位点的准确性,试验采用obsERVer流程和位点特异性PCR方法,测定了3只BWEL-SPF鸡全基因组测序数据中的ALVE基因插入位点,并验证了obsERVer流程预测的准确性。结果表明:在3只BWEL-SPF鸡中共识别到4个ALVE基因插入位点,分别为ALVE1、ALVE3、ALVE9和ALVE15;插入位点上下游序列存在规律性,是由6个碱基的基序构成的重复序列;并且这4个插入位点均可以通过位点特异性PCR方法验证,吻合率为100%。说明obsERVer流程可以准确预测测序数据中的ALVE基因插入位点,在BWEL-SPF鸡中存在多个ALVE基因插入位点。To verify the accuracy of bioinformatic methods identifying Avian leukosis virus subgroup E(ALVE)insertion sites,the obsERVer pipeline and locus-specific PCR were used to determine the insertion sites of ALVE gene in the whole genome sequencing data of three BWEL-SPF chickens,and the accuracy of the prediction of obsERVer process was verified.The results showed that there were four ALVE insertion sites identified in BWEL-SPF chickens.The ALVE insertion sites were ALVE1,ALVE3,ALVE9 and ALVE15.The sequence upstream and downstream of the insertion site was regular,which was a repetitive sequence composed of 6 bases.These ALVE insertion sites could be verified by locus-specific PCR,and the coincidence rate was 100%.These results indicated that the obsERVer process could accurately predict the insertion sites of ALVE gene in sequencing data,and there were various insertion sites of ALVE gene in BWEL-SPF chickens.

关 键 词：BWEL-SPF鸡 禽白血病病毒E亚群 obsERVer流程 全基因组重测序数据 插入位点 

分 类 号：S852.659.3[农业科学—基础兽医学] Q754[农业科学—兽医学]