雷帕霉素对肺癌SPC-A-1细胞增殖和凋亡的影响  

Effect of rapamycin on proliferation and apoptosis of human lung cancer SPC-A-1 cells

作　　者：孙潺[1] 张轩斌[1] 赵江[1] 代岩[1] SUN Chan;ZHANG Xuan-Bin;ZHAO Jiang;DAI Yan(Department of respiratory,Center Hospital of Nanyang City,Nanyang,Henan 473000,China)

机构地区：[1]南阳市中心医院呼吸二科,河南南阳473000

出　　处：《临床肺科杂志》2023年第6期829-833,共5页Journal of Clinical Pulmonary Medicine

基　　金：北京医卫健康公益基金会(YWJKJJHKYJJ-F1002B)。

摘　　要：目的分析不同浓度的雷帕霉素体外处理人肺癌SPC-A-1细胞后,对细胞的增殖及凋亡的影响,并探讨其可能的作用机制。方法采用CCK-8法测定雷帕霉素(5 ng/mL,10 ng/mL,15 ng/mL)处理人肺癌SPC-A-1细胞48 h后对细胞增殖的影响。运用FITC-Annexin V/PI双染色法,测定雷帕霉素处理SPC-A-1细胞48 h后对细胞凋亡的影响。使用Western Blot技术测定雷帕霉素作用SPC-A-1细胞48h后的p53、Bcl-2和Bax等凋亡蛋白表达情况。结果雷帕霉素可以抑制SPC-A-1细胞增殖并可诱导其发生凋亡,其增殖抑制率与凋亡率变化存在剂量依赖性关系(P<0.05)。与对照组(雷帕霉素,0 ng/mL)比较,各浓度(5 ng/mL,10 ng/mL,15 ng/mL)雷帕霉素处理对SPC-A-1细胞的凋亡率上升(P<0.05),且p53与Bax蛋白水平增加(P<0.05),而Bcl-2蛋白水平下降(P<0.05)。15 ng/mL雷帕霉素处理组细胞p53、Bax蛋白表达水平均与细胞凋亡率呈正相关(r=0.906,P<0.05;r=0.938,P<0.05);Bcl-2蛋白表达水平与细胞凋亡率呈负相关(r=-0.712,P<0.05)。结论雷帕霉素可抑制人肺癌SPC-A-1细胞发生增殖并可促进其发生凋亡,其作用机制可能与雷帕霉素可以上调p53、Bax蛋白表达水平和下调Bcl-2蛋白表达水平相关。Objective To analyze the effects of different concentrations of rapamycin on the proliferation and apoptosis of human lung cancer SPC-A-1 cells in vitro,and to explore the possible mechanism.Methods The effects of rapamycin(5 ng/mL,10 ng/mL,15 ng/mL)on the proliferation of human lung cancer SPC-A-1 cells for 48 h were determined by a CCK-8 assay.Annexin V-FITC and PI double staining was used to determine the cell apoptosis.Western blot was used to detect the protein expression of p53,Bcl-2,and Bax.Results Rapamycin treatment could significantly inhibit cell proliferation and induce apoptosis of SPC-A-1 cells in a dose-dependent manner(P<0.05).Compared with the control group(rapamycin,0 ng/mL),the apoptotic rate in SPC-A-1 cells treated with different concentrations of rapamycin was markedly increased(P<0.05),accompanied by the up-regulation of protein levels of p53 and Bax while the down-regulation of Bcl-2(P<0.05).In the 15 ng/mL rapamycin-treated group,the expression levels of p53 and Bax protein were all positively correlated with the apoptosis rate(r=0.906,P<0.05;r=0.938,P<0.05,respectively),while the level of Bcl-2 protein was negatively correlated with the apoptosis rate(r=-0.712,P<0.05).Conclusion Rapamycin treatment can inhibit proliferation and promote apoptosis of human lung cancer SPC-A-1 cells,and the underlying mechanism may be related to the up-regulation of p53,Bax,and the down-regulation of Bcl-2 protein expression.

关 键 词：肺癌 雷帕霉素 细胞增殖 细胞凋亡 

分 类 号：R734.2[医药卫生—肿瘤]