PCR技术在疱疹病毒性角膜炎诊断及指导治疗中的应用  

Application of polymerase chain reaction in diagnosis and guiding treatment of human herpesvirus keratitis

作　　者：王丹丹 许叶圣[1] 郑利斌 姚玉峰[1] Wang Dandan;Xu Yesheng;Zheng Libin;Yao Yufeng(Department of Ophthalmology,Sir Run Run Shaw Hospital,Zhejiang University School of Medicine,Zhejiang Province Key Laboratory of Corneal Diseases Research,Hangzhou 310018)

机构地区：[1]浙江大学医学院附属邵逸夫医院眼科、浙江省角膜病研究重点实验室,杭州310018

出　　处：《国际眼科纵览》2023年第2期181-185,共5页International Review of Ophthalmology

基　　金：国家自然科学基金(U20A20387);浙江省医药卫生科技计划项目(2019ZD040)。

摘　　要：人类疱疹病毒是引起病毒性角膜炎最常见的病原体,聚合酶链反应(polymerase chain reaction,PCR)通过扩增目的基因,能在有限的眼部标本中快速、准确检测出可疑病原体。PCR技术经优化后更适合于临床检测,其中多重PCR可同时鉴别多种病毒亚型,所需标本量小且效率更高;实时荧光定量PCR可检测病毒载量,提示是否出现耐药以及是否可以停药;固相PCR减少移液误差,检测结果更为快速、稳定。深入了解各类PCR技术及其应用有助于疱疹病毒性角膜炎的准确诊断以及治疗方案的制定。Human herpes virus is the most common pathogen causing viral keratitis.Polymerase chain reaction(PCR)technology can rapidly and accurately detect suspected pathogens in a limited number of eye samples by amplifying target genes.After optimization,PCR technology is more suitable for clinical detection.Multiplex PCR can identify various virus subtypes at the same time,which is more efficiency and requires less sample.Quantitative real-time PCR can detect viral load,which can be used to guide whether drug resistance occurs during the treatment process and whether to stop the medicine.Solid-phase PCR can reduce the pipetting error,and the detection results are more rapid and stable.A thorough understanding of various PCR techniques and their applications will help to better diagnose and guide the treatment of herpes virus-associated keratitis.

关 键 词：人类疱疹病毒 疱疹病毒性角膜炎 聚合酶链反应 

分 类 号：R772.21[医药卫生—眼科]