两种外生菌根真菌的分离鉴定及其培养特性研究  

Isolation and identification of two ectomycorrhizal fungi and study of their culture characteristics

作　　者：孙启彪 钱璐 何贞英 欧阳建萍 何芬 季晓红 陈晔[1] 何刚 Sun Qibiao;Qian Lu;He Zhenying;Ouyang Jianping;He fen;Ji Xiaohong;Chen Ye;He Gang(Jiujiang Key Laboratory of Fungal Resources Conservation and Utilization,College of Pharmacy and Life Sciences,Jiujiang University,Jiujiang Jiangxi 332000,China;College of Resources&Environment,Jiujiang University,Jiujiang Jiangxi 332005,China)

机构地区：[1]九江学院·药学与生命科学学院,九江市真菌资源保育及应用重点实验室,江西九江332000 [2]九江学院·资源环境学院,江西九江332005

出　　处：《南方林业科学》2023年第2期47-52,共6页South China Forestry Science

基　　金：国家自然科学基金项目(项目编号:32260031,31900018);江西省自然科学基金项目(项目编号:20202BAB213025);江西省大学生创新创业训练计划项目(项目编号:s202111843041,s202111843052)。

摘　　要：为获取外生菌根真菌种质资源,了解其离体培养最适条件,本研究对采自江西省九江市庐山国家级自然保护区的两种外生菌根真菌子实体进行组织分离,结合子实体形态和ITS序列对两种外生菌根真菌进行鉴定,采用固体培养法研究温度、pH、碳源、氮源及碳氮比对两种外生菌根真菌生长的影响。结果表明,两种外生菌根真菌经鉴定分别为柳氏硬皮地星(Astraeus ryoocheoninii)和花脸香蘑(Lepista sordida)。在纯培养条件下,两株真菌的最适温度均为25℃,最适生长pH值为5-6,最适碳源均为葡萄糖,最适氮源均为铵盐,柳氏硬皮地星最适碳氮比为5∶1,花脸香蘑最适碳氮比为40∶1。本研究初步确定了这两种ECM真菌的最适培养条件,为其菌丝体扩繁和菌剂生产及开发其食药用价值和生态价值奠定了基础。In order to obtain the germplasm resources of ectomycorrhizal(ECM)fungi and understand the most suitable conditions for culture in vitro.The fruiting bodies of two ECM fungi collected from Lushan National Nature Reserve in Jiujiang,Jiangxi Province were isolated by tissue separation,and the two ECM fungi were identified by combining fruiting body morphology and ITS sequence,and the effects of temperature,pH,carbon source,nitrogen source and C/N ratio on the growth of two ECM fungi were studied by solid culture method.Two ECM fungi have been identified as Astraeus ryoocheoninii and Lepista sordida,respectively.Under pure culture conditions,the optimal temperature of both fungi was 25℃,the optimal growth pH was 5-6,the most suitable carbon source was glucose,the most suitable nitrogen sources were ammonium salts,the optimal C/N ratio of A.ryoocheoninii was 5∶1,and the optimal C/N ratio of L.sordida was 40∶1.This study preliminarily determined the optimal culture conditions for these two ECM fungi,which supply a foundation for their mycelial propagation and the inoculum production of ECM fungi and the development of their edible,medicinal and ecological values.

关 键 词：外生菌根真菌 柳氏硬皮地星 花脸香蘑 ITS序列 培养条件 

分 类 号：S759.81[农业科学—森林经理学] Q949.32[农业科学—林学]