野菊对α-葡萄糖苷酶的抑制作用及其相关化学成分研究  

Study on the inhibition of α -glucosidase by Chrysanthemum indium L.and its related chemical constituents

作　　者：苏健[1] 凌兰鑫 张桂珍 江圆圆 黄春韵 胡颖 SU Jian;LING Lan-xing;ZHANG Gui-zhen;JIANG Yuan-yuan;HUANG Chun-yun;HU Ying(Guangxi Scientific Research Center of Traditional Chinese Medicine,Guangxi University of Chinese Medicine,Nanning 530200,China;Faculty of Pharmacy,Guangxi University of Chinese Medicine,Nanning 530200,China)

机构地区：[1]广西中医药大学广西中医药科学实验中心,广西南宁530200 [2]广西中医药大学药学院,广西南宁530200

出　　处：《云南民族大学学报（自然科学版）》2023年第2期138-144,共7页Journal of Yunnan Minzu University:Natural Sciences Edition

基　　金：广西壮瑶药重点实验室(桂科基字【2014】32号);壮瑶药协同创新中心(桂教科研【2013】20号);广西中药一流学科中药学(民族医药方向)项目(桂教科研【2018】12号);广西高校中药药理重点实验室(Jl700304).

摘　　要：以α-葡萄糖苷酶为靶点,研究野菊降糖作用机制,测试野菊提取物对其活性抑制作用,并筛选出相关化合物.用系统溶剂提取法制备野菊花、叶、根和茎的提取物,进行α-葡萄糖苷酶抑制实验,确定活性部位;用液相-高分辨质谱分析各提取物的化学成分差异,筛选在活性部位高表达的化合物.野菊花的正丁醇、乙酸乙酯和二氯甲烷部位,根的正丁醇和二氯甲烷部位,茎的乙酸乙酯、二氯甲烷和正己烷部位IC 50小于10μg/mL,经质谱分析从这些活性部位中筛选出高表达的19个化合物.结果表明野菊植物中含有抑制α-葡萄糖苷酶活性的化合物;质谱分析中得到的19个潜在相关的化合物,可作为进一步研究的基础.In this study,α-glucosidase was used as the target to study the hypoglycemic mechanism of Chrysanthemum indium L.,test the inhibitory effect of Chrysanthemum indium L.extract on its activity,and screen out related compounds.The extracts from Chrysanthemum indium L.flowers,leaves,roots and stems were prepared by systematic solvent extraction,and theα-glucosidase inhibition experiment was carried out to determine the active parts.Liquid chromatography-high resolution mass spectrometry was used to analyze the differences in chemical composition of the extracts,and the compounds highly expressed in active parts were screened.The IC 50 of n-butanol,ethyl acetate and dichloromethane in flower,n-butanol and dichloromethane in root,ethyl acetate,dichloromethane and n-hexane in stem were less than 10μg/mL.19 compounds with high expression were screened from these active parts by mass spectrometry.The results show that Chrysanthemum indium L.plants contain compounds that inhibit the activity ofα-glucosidase.The 19 potentially related compounds obtained from mass spectrometry analysis can serve as a basis for further research.

关 键 词：野菊 Α-葡萄糖苷酶 抑制作用 化学成分 

分 类 号：R285[医药卫生—中药学]