过表达CBX7调控PTEN/Akt信号通路干预上皮-间充质转化抑制胃癌细胞迁移、侵袭  

Overexpression of CBX7 inhibits the migration and invasion of gastric cancer cells by regulating PTEN/Akt signaling pathway to interfere epithelial-mesenchymal transition

作　　者：谭斌[1] 刘剑波[1] 李芳芳[1] 彭艳 钟月圆 邓晖[1] 王秀丽 TAN Bin;LIU Jian-bo;LI Fang-fang;PENG Yan;ZHONG Yue-yuan;DENG Hui;WANG Xiu-li(Department of Gastroenterology,Central Hospital of First People's Hospital of Chenzhou,Chenzhou Hunan 423000,China)

机构地区：[1]郴州市第一人民医院中心医院消化内科,湖南郴州423000

出　　处：《局解手术学杂志》2023年第5期394-399,共6页Journal of Regional Anatomy and Operative Surgery

摘　　要：目的探讨染色体盒蛋白同源物7(CBX7)对胃癌细胞上皮-间充质转化(EMT)、迁移、侵袭的影响及相关作用机制。方法将CBX7过表达质粒和阴性对照质粒、CBX7 siRNA和阴性对照siRNA转染至体外培养的人胃癌细胞SGC7901。采用qRT-PCR和Western blot法检测细胞中CBX7的mRNA和蛋白表达,CCK-8法检测细胞增殖情况,细胞划痕实验检测细胞迁移情况,Transwell小室检测细胞侵袭情况,Western blot法检测细胞中EMT相关蛋白和磷酸酶张力蛋白同源物基因(PTEN)/蛋白激酶B(Akt)信号通路相关蛋白表达。结果与pcDNA-NC组比较,pcDNA-CBX7组SGC7901细胞存活率、迁移率及侵袭数均显著下降/减少,细胞中E-钙黏蛋白(E-cadherin)、PTEN蛋白表达量均显著升高,N-钙黏蛋白(N-cadherin)和波形蛋白(Vimentin)蛋白表达量、p-Akt/Akt均显著下降,差异有统计学意义(P<0.05)。与si-NC组比较,si-CBX7组SGC7901细胞存活率、迁移率及侵袭数均显著升高/增加,细胞中E-cadherin、PTEN蛋白表达量均显著下降,N-cadherin、Vimentin蛋白表达量和p-Akt/Akt均显著升高,差异有统计学意义(P<0.05)。结论过表达CBX7可通过抑制EMT进程抑制胃癌细胞迁移、侵袭,其作用机制可能与调控PTEN/Akt信号通路有关。Objective To investigate the effects and related mechanisms of chromosome box protein homologue 7(CBX7)on epithelialmesenchymal transition(EMT),migration and invasion in gastric cancer cells.Methods The CBX7 overexpression plasmid and negative control plasmid,CBX7 siRNA and negative control siRNA were transfected into human gastric cancer cells SGC7901 cultured in vitro.The mRNA and protein expression of CBX7 in cells were detected by qRT-PCR and Western blot,the cell proliferation was detected by CCK-8 method,the cell migration was detected by scratch wound healing assay,the cell invasion was detected by Transwell assay,the expression of cell EMT related proteins and phosphatase tension protein homologue gene(PTEN)/protein kinase B(Akt)signaling pathway related proteins were detected by Western blot.Results Compared with the pcDNA-NC group,the survival rate,migration rate and invasion number of SGC7901 cells in the pcDNA-CBX7 group were significantly decreased,the protein expression of E-cadherin and PTEN in cells were significantly increased,and the protein expression of N-cadherin,Vimentin and the ratio of p-Akt/Akt were significantly decreased,with statistically significant differences(P<0.05).Compared with the si-NC group,the survival rate,migration rate and invasion number of SGC7901 cells in the si-CBX7 group were significantly increased,the protein expression of E-cadherin and PTEN in cells were significantly decreased,and the protein expression of N-cadherin,Vimentin and the ratio of p-Akt/Akt were significantly increased,with statistically significant differences(P<0.05).Conclusion Overexpression of CBX7 can inhibit the migration and invasion of gastric cancer cells by inhibiting the EMT process,and its mechanism may be related to the regulation of PTEN/Akt signaling pathway.

关 键 词：染色体盒蛋白同源物7 胃癌 上皮-间充质转化 迁移 侵袭 PTEN/Akt信号通路 

分 类 号：R735.2[医药卫生—肿瘤]