缺氧预适应BMSC-EVs在大鼠肠道细胞H_(2)O_(2)损伤模型中的初步研究  

Preliminary study of hypoxia-preconditioned BMSC-EVs in rat intestinal cell H_(2)O_(2) injury model

作　　者：吴柯[1] 赵珺[2] 咸华[2] WU Ke;ZHAO Jun;XIAN Hua(School of Medicine,Nantong University,Nantong 226001;Department of Pediatric Surgery,the Affiliated Hospital of Nantong University)

机构地区：[1]南通大学医学院,南通226001 [2]南通大学附属医院小儿外科

出　　处：《南通大学学报（医学版）》2023年第2期107-112,共6页Journal of Nantong University(Medical sciences)

基　　金：江苏省研究生科研与实践创新计划项目(SJCX20_1158),南通市社会民生面上项目(MS12021007)。

摘　　要：目的:探索缺氧预适应骨髓间充质干细胞(bone marrow mesenchymal stem cells,BMSCs)来源的囊泡(extracellular vesicles,EVs)能否在大鼠肠道细胞(IEC-6)H_(2)O_(2)损伤模型中发挥积极作用。方法:培养BMSCs,并进行缺氧培养48 h,分离获取BMSC-EVs。培养IEC-6,构建H_(2)O_(2)损伤模型,分为Normal组、H_(2)O_(2)组和H_(2)O_(2)+EVs组。采用CCK-8活力检测和EdU染色观察缺氧预适应BMSC-EVs对IEC-6损伤后增殖的影响;划痕实验检测缺氧预适应BMSC-EVs对IEC-6损伤后迁移的影响;通过实时定量PCR,从基因水平检测不同组别炎症因子IL-6、TNF-α和凋亡因子Caspase-3的变化。结果:CCK-8活力检测和EdU染色结果显示缺氧预适应BMSC-EVs可以促进IEC-6损伤后增殖;划痕实验结果显示缺氧预适应BMSC-EVs促进IEC-6损伤后迁移;实时定量PCR发现缺氧预适应BMSC-EVs处理后的细胞炎症因子IL-6、TNF-α和凋亡因子Caspase-3较未处理组降低。结论:缺氧预适应BMSC-EVs可以促进IEC-6损伤后的增殖和迁移,并可抑制IEC-6损伤后的炎症因子和凋亡因子的表达。Objective:To explore whether hypoxia preconditioning bone marrow mesenchymal stem cells(BMSCs)-derived extracellular vesicles(EVs)can play a positive role in the H2O2 injury model of rat intestinal cells(IEC-6).Methods:BMSCs were cultured and cultured in hypoxia for 48 hours,and BMSC-EVs were isolated and obtained.IEC-6 was cultured to construct H_(2)O_(2) injury model,which was divided into Normal group,H_(2)O_(2) group and H_(2)O_(2)+EVs group.CCK-8 activity test and EdU staining were used to observe the effect of hypoxia preconditioning BMSC-EVs on proliferation after IEC-6 injury.Scratch test was used to detect the role of hypoxia preconditioning BMSC-EVs in promoting the migration of IEC-6 after injury.The changes of inflammatory factors IL-6,TNF-αand apoptotic factor Caspase-3 in different groups were detected at the gene level by real-time quantitative PCR.Results:CCK-8 activity test and EdU staining observed that hypoxia preconditioning BMSC-EVs could promote the proliferation of IEC-6 after injury.Scratch test detected that hypoxia preconditioning BMSC-EVs promoted the migration of IEC-6 after injury.Through real-time quantitative PCR,it was found that the inflammatory factors IL-6,TNF-αand apoptosis factor Caspase-3 of hypoxia preconditioning BMSC-EVs were lower than those of untreated group.Conclusion:Hypoxia preconditioning BMSC-EVs can promote the proliferation and migration after IEC-6 injury,and inhibit the expression of inflammatory factors and apoptotic factors after IEC-6 injury.

关 键 词：骨髓间充质干细胞 缺氧预适应 囊泡 肠缺血再灌注损伤 

分 类 号：R574[医药卫生—消化系统]