As_(2)O_(3)对大鼠BRL-3A和RH-35细胞TRβ1和Cyclin D1因子的影响  

Effects of As_(2)O_(3)on TRβ1and Cyclin D1factors in rat BRL-3Aand RH-35cells

作　　者：王炼[1,2] 王雪飞[1] 刘丹 耿敏杰[4] 郭志伟 崔娜 刘建平 夏雅娟 杨英 WANG Lian;WANG Xuefei;LIU Dan;GENG Minjie;GUO Zhiwei;CUI Na;LIU Jianping;XIA Yajuan;YANG Ying(College of Veterinary Medicine,Henan University of Animal Husbandry and Economy,Zhengzhou 450000,China;College of Veterinary Medicine,Inner Mongolia Agricultural University,Hohhot 010018,China;Bayannaoer City Center for Disease Control and Prevention,Bayannaoer,Inner Mongolia 014000,China;Baotou City Primary Health Service Guidance Center,Baotou,Inner Mongolia 014010,China;Institute of Endemic Disease and Chronic Disease,Inner Mongolia Center for Disease Control and Prevention,Hohhot 010031,China)

机构地区：[1]河南牧业经济学院动物医药学院,河南郑州450000 [2]内蒙古农业大学兽医学院,内蒙古呼和浩特010018 [3]内蒙古巴彦淖尔市疾病预防控制中心,内蒙古巴彦淖尔014000 [4]内蒙古包头市疾病预防控制中心,内蒙古包头014010 [5]内蒙古地方病防治研究中心,内蒙古呼和浩特010031

出　　处：《中国兽医学报》2023年第4期741-747,共7页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(81360414);河南省科技发展计划资助项目(222102110211);河南省教育厅重点研发资助项目(21B230007)。

摘　　要：为了探明As_(2)O_(3)对大鼠肝脏细胞系BRL-3A和肝癌细胞系RH-35中TH信号通路的关键调控因子TRβ1和TRβ1的下游因子Cyclin D1的影响,采用qRT-PCR、Western blot技术检测2种细胞中TRβ1和Cyclin D1的基因和蛋白表达变化。结果显示:(1)与对照组相比,As_(2)O_(3)作用BRL-3A细胞12h,1.563μmol/L组TRβ1蛋白表达显著降低(P<0.01)、Cyclin D1表达显著升高(P<0.01);6.250μmol/L组TRβ1表达明显降低(P<0.05)、Cyclin D1表达显著降低(P<0.01)。As_(2)O_(3)作用24h,1.563μmol/L组TRβ1蛋白表达明显升高(P<0.05);6.250μmol/L组TRβ1显著升高(P<0.01),Cyclin D1的表达显著降低(P<0.01)。As_(2)O_(3)作用48h,6.250μmol/L组TRβ1蛋白表达显著升高(P<0.01),各组Cyclin D1表达均显著降低(P<0.01)。基因表达结果与蛋白表达结果基本一致。(2)与对照组相比,As_(2)O_(3)作用RH-35细胞12h,各组TRβ1蛋白表达量显著升高(P<0.01)。As_(2)O_(3)作用RH-3524h,1.563μmol/L组TRβ1蛋白表达量明显升高(P<0.05),其余组显著升高(P<0.01)。As_(2)O_(3)作用48h,3.125和6.250μmol/L组TRβ1蛋白表达显著升高(P<0.01),1.563μmol/L组明显降低(P<0.05)。As_(2)O_(3)作用RH-3512,24,48h,各组Cyclin D1蛋白表达量均显著降低(P<0.01)。基因结果与蛋白结果基本一致。表明As_(2)O_(3)随着作用时间的增加,引起大鼠肝脏细胞系BRL-3A和肝癌细胞系RH-35TRβ1的水平异常,进而干扰Cyclin D1的水平。In order to explore the effect of As_(2)O_(3)on the key regulators TRβ1and Cyclin D1of TH signaling pathway in rat liver cell line BRL-3Aand liver cancer cell line RH-35,qRT-PCR and Western blot techniques were used to detect the expression changes of genes and protein of TRβ1 and Cyclin D1.The results indicate that:(1)Compared with the control group,the expression of TRβ1protein in the 1.563μmol/L group was reduced(P<0.01)and the expression of Cyclin D1 protein was increased(P<0.01)in the BRL-3Acells treated with As_(2)O_(3)for 12h.The expression of TRβ1protein in the 6.250μmol/L group was decreased(P<0.05),Cyclin D1expression was decreased(P<0.01).After treatment with As_(2)O_(3)for 24h,TRβ1protein increased in the 1.563μmol/L group(P<0.05)and increased in the 6.250μmol/L group(P<0.01),and the expression of Cyclin D1protein decreased(P<0.01).After 48hof treatment with As_(2)O_(3),the expression of TRβ1protein in the 6.250μmol/L group was increased(P<0.01),and the protein expression of Cyclin D1in each group was decreased(P<0.01).The gene expression results were basically consistent with the protein expression results.(2)Compared with the control group,As_(2)O_(3)treated RH-35cells for 12h,the expression of TRβ1protein in each group was increased(P<0.01).As_(2)O_(3)treated RH-35for 24h,the expression of TRβ1protein in the 1.563μmol/L group was increased(P<0.05)and the other groups were increased(P<0.01).After As_(2)O_(3)theatment for 48h,the expression of TRβ1protein in the 3.125and 6.250μmol/L groups were increased(P<0.01),and the 1.563μmol/L group was decreased(P<0.05).Compared with the control group,As_(2)O_(3)treated RH-35cells for 12,24and 48h,the Cyclin D1protein expression in each group was reduced(P<0.01).The gene results were basically consistent with the protein results.It showed that As_(2)O_(3)caused abnormal levels of TRβ1in rat liver cell line BRL-3Aand liver cancer cell line RH-35with the increase of action time,and then interfered with the level of Cyclin D1.

关 键 词：三氧化二砷 大鼠肝脏细胞系BRL-3A 大鼠肝癌细胞系RH-35 甲状腺激素受体β1 细胞周期蛋白D1 

分 类 号：R737.9[医药卫生—肿瘤]