趋化因子CXCL11与其受体CXCR3在口腔鳞状细胞癌中的表达及临床意义  

Expressions and Clinical Significance of CXCL11 and Its Receptor CXCR3 in Oral Squamous Cell Carcinoma

作　　者：李勇[1] 李全[1] 乔飞 刁艳菲 黄云生 戚晓峰 安莉 Li Yong;Li Quan;Qiao Fei;Diao Yanfei;Huang Yunsheng;Qi Xiaofeng;An Li(Department of Stomatology,Second Affiliated Hospital of Suzhou University,Suzhou 215004,Jiangsu,China;Affiliated Stomatological Hospital of Suzhou Health Vocational and Technical College,Suzhou 215002,Jiangsu,China)

机构地区：[1]苏州大学附属第二医院口腔科,江苏苏州215004 [2]苏州卫生职业技术学院附属口腔医院牙体牙髓病科,江苏苏州215002

出　　处：《肿瘤预防与治疗》2023年第4期288-295,共8页Journal of Cancer Control And Treatment

基　　金：国家自然科学基金(编号:81922025);苏州市科技发展计划项目(编号:SKJYD2021051)。

摘　　要：目的:研究CXC型趋化因子配体11(C-X-C-motif chemokine ligand 11,CXCL11)及CXC趋化因子受体3(C-X-C-motif chemokine receptor 3,CXCR3)在口腔鳞状细胞癌(oral squamous cell carcinoma,OSCC)组织中的表达及临床意义。方法:在GEO数据库中下载GSE30784数据集筛选差异性表达基因,进行GO(Gene Ontology)富集分析。搜集67例不同分化程度的OSCC组织和28例正常口腔黏膜组织,采用免疫组化方法分别检测CXCL11和CXCR3两种蛋白的表达,分析CXCL11和CXCR3与OSCC临床病理特征及两者之间的相关性,并结合随访资料分析CXCL11和CXCR3与生存期之间的关系。结果:GEO数据集分析发现CXCL11表达在OSCC组织中显著上调(LogFC=3.545,P<0.001),GO富集分析发现其富集于肿瘤细胞侵袭和迁移中;CXCL11和CXCR3在OSCC组织中的阳性表达率分别为68.7%及74.6%,显著高于正常口腔黏膜组织(P<0.05);CXCL11和CXCR3在OSCC中的表达与肿瘤分化程度、临床分期、淋巴结转移与否有关(P<0.05),与年龄、性别、肿瘤直径的大小均无关(P>0.05);OSCC中,CXCL11和CXCR3的表达可能相关(r=0.271,P=0.026);CXCL11、CXCR3阳性表达患者生存期可能短于CXCL11、CXCR3阴性表达患者(P<0.05)。结论:CXCL11和CXCR3在口腔鳞癌中过表达,可能参与OSCC的发生、发展,并导致不良预后,为OSCC的早期诊断、预后评估以及靶向治疗提供新的依据。Objective:To investigate the expressions and clinical significance of C-X-C motif chemokine ligand 11(CXCL11)and C-X-C motif chemokine receptor 3(CXCR3)in oral squamous cell carcinoma(OSCC)tissue.Methods:We downloaded the GSE30784 dataset in the Gene Expression Omnibus(GEO)database to screen differentially expressed genes for GO enrichment analysis.67 cases of OSCC tissue with different degrees of differentiation and 28 cases of normal oral mucosal tissue were collected.The expressions of CXCL11 and CXCR3 proteins were detected by immunohistochemistry.The relationship between CXCL11/CXCR3 expression and the clinicopathological features of OSCC,and the correlation between CXCL11 and CXCR3 expressions were analyzed.And the relationship between CXCL11/CXCR3 expression and survival was analyzed based on follow-up data.Results:GEO dataset analysis showed that CXCL11 expression was significantly up-regulated in OSCC tissue(LogFC=3.545,P 0.001);GO enrichment analysis found that CXCL11 was enriched in tumor cell invasion and migration.The positive expression rates of CXCL11 and CXCR3 in OSCC tissue were 68.7%and 74.6%,respectively,which were significantly higher than those in normal oral mucosal tissue(P<0.05).The expressions of CXCL11 and CXCR3 in OSCC were related to the degree of differentiation,clinical stage and lymph node metastasis(r=0.271,P=0.05),and did not affect age,sex or tumor diameter(P<0.05).The expressions of CXCL11 and CXCR3 in OSCC might be correlated(P<0.05);In OSCC patients,the survival time of CXCL11-and CXCR3-positive groups might be shorter than that of CXCL11-and CXCR3-negative groups(P<0.05).Conclusion:The over-expressions of CXCL11 and CXCR3 in OSCC may participate in the occurrence and development of OSCC,and lead to poor prognosis,which provides new evidence for early diagnosis,prognosis assessment and targeted treatment of OSCC.

关 键 词：CXCL11 CXCR3 口腔鳞状细胞癌 免疫组织化学 生物信息学 

分 类 号：R739.8[医药卫生—肿瘤]