Metagenomic insights into responses of microbial population and key functional genes to fulvic acid during partial nitritation  

作　　者：Li Zhang Shuang Lan Quanhao Dou Shiwei Hao Yueping Wang Xiaoxuan Wang Ruoyan Zhang Yongzhen Peng Jiachun Yang 

机构地区：[1]National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology,Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering,Beijing University of Technology,Beijing 100124,China [2]Shuifa Shandong Water Development Group Co.Ltd.,Shandong 274200,China

出　　处：《Journal of Environmental Sciences》2023年第2期952-962,共11页环境科学学报（英文版）

基　　金：supported by the Key Research&Developmental Program of Shandong Province(Nos.2021CXGC011202,2020CXGC011404);the Beijing Natural Science Foundation(No.8192004);the Beijing Major Science and Technology Projects(No.Z181100005318001)

摘　　要：The long-term impact of fulvic acid(FA)on partial nitritation(PN)systemwas initially examined in this study.The obtained results revealed that the FA lower than 50 mg/L had negligible effect on the nitrite accumulation rate(NAR nearly 100%)and ammonium removal rate(ARR 56.85%),while FA over 50 mg/L decreased ARR from 56.85%to 0.7%.Sludge characteristics analysis found that appropriate FA(<50 mg/L)exposure promoted the settling performance and granulation of PN sludge by removing Bacteroidetes and accumulating Chloroflexi.The analysis of metagenomics suggested that the presence of limited FA(0-50 mg/L)stimulated the generation of NADH,which favors the denitrification and nitrite reduction.The negative impact of FA on the PN system could be divided into two stages.Initially,limited FA(50-120 mg/L)was decomposed by Anaerolineae to stimulate the growth and propagation of heterotrophic bacteria(Thauera).Increasing heterotrophs competed with AOB(Nitrosomonas)for dissolved oxygen,causing AOB to be eliminated and ARR to declined.Subsequently,when FA dosage was over 120 mg/L,Anaerolineae were inhibited and heterotrophic bacteria reduced,resulting in the abundance of AOB recovered.Nevertheless,the ammonium transformation pathway was suppressed because genes amoABC and hao were obviously reduced,leading to the deterioration of reactor performance.Overall,these results provide theoretical guidance for the practical application of PN for the treatment of FA-containing sewage.

关 键 词：Partial nitritation Fulvic acid Microbial community Functional genes METAGENOMIC 

分 类 号：X172[环境科学与工程—环境科学] X703