丹参多酚酸盐通过SIRT3/β-catenin-PPARγ信号通路对心肌梗死大鼠的作用及机制研究  

Effect and Mechanism of Salvianolate on Myocardial Infarction Rats through SIRT3/β-catenin-PPARγSignaling Pathway

作　　者：张文强 李慧 朱芳红 叶飞林 薛强 ZHANG Wen-qiang;LI Hui;ZHU Fang-hong;YE Fei-lin;XUE Qiang(Department of Cardiology,986 Air Force Hospital,Xi'an,Shaanxi,710054,China;Department of Cardiology,Xijing Hospital of Air Force Medical University,Xi'an,Shaanxi,710032,China)

机构地区：[1]空军第九八六医院心内科,陕西西安710054 [2]空军军医大学西京医院心内科,陕西西安710032

出　　处：《现代生物医学进展》2023年第7期1225-1230,共6页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(82104718)。

摘　　要：目的:通过制备心肌梗死大鼠模型,基于SIRT3/β-catenin-PPARγ信号通路探讨丹参多酚酸盐对心肌梗死大鼠的作用及相关机制,为将丹参多酚酸盐应用于心肌梗死治疗积累理论基础。方法:选取SPF级健康SD大鼠60只,随机分为假手术组(A组)、模型组(B组)、丹参多酚酸注射低剂量组(C组)丹参多酚酸注射高剂量组(D组)。B、C、D组大鼠制备为心肌梗死模型,A组大鼠仅进行假手术操作。B、C、D三组大鼠均接受丹参多酚酸盐注射治疗,A组大鼠注射等量生理盐水。比较各组大鼠心动图指标、血流动力学参数、心肌损伤相关指标、SIRT3/β-catenin-PPARγ信号通路相关蛋白及m RNA相对表达量。结果:干预后,B、C、D组大鼠的LVEF、LVFS和dP/dt max、dP/dt min均下降,且C、D组高于B组,D组高于C组;同时B、C、D组大鼠LVEDD高于A组,C、D组低于B组且D组低于C组。B、C、D组大鼠CK、CK-MB、LDH水平均高于A组,同时C、D组均低于B组,且D组低于C组;干预后,B、C、D组大鼠IL-1β、IL-6、TNF-α水平均高于A组,同时C、D组均低于B组,且D组低于C组;干预后,B、C、D组大鼠SIRT3、β-catenin蛋白表达量及m RNA表达量均高于A组,同时C、D组大鼠高于B组且D组高于C组;同时B、C、D组PPARγ蛋白表达量及m RNA表达量均高于A组,且C、D组低于B组,D组低于C组(P<0.05)。结论:丹参多酚酸盐能够有效改善心肌梗死大鼠的心功能,缓解心肌损伤,其机制可能与调控SIRT3/β-catenin-PPARγ信号通路、下调炎性因子水平相关,且高剂量丹参多酚酸盐的治疗效果更优。Objective:To establish a rat model of myocardial infarction,and explore the effect of salvianolate on myocardial infarction and its related mechanism based on SIRT3/β-catenin-PPARγsignaling pathway,which can provide the theoretical basis for the application of salvianolate in the treatment of myocardial infarction.Methods:60 SPF healthy SD rats were randomly divided into sham operation group(group A),model group(group B),low-dose group(group C)and high-dose group(group D).Rats in groups B,C and D were made into myocardial infarction models,while rats in group A only underwent sham operation.Rats in groups B,C and D were treated by injection of salvianolate,while rats in group A were injected with the same amount of normal saline.The indexes of echocardiography,hemodynamic parameters,myocardial injury related indexes,SIRT3/β-catenin-PPARγsignal pathway related protein and mRNA relative expression were compared in each group.Results:After intervention,the LVEF,LVFS,dP/dt max,dP/dt min of rats in groups B,C and D decreased,and those in groups C and D were higher than those in group B,and those in group D were higher than those in group C.Meanwhile,LVEDD was higher in groups B,C and D than in group A,lower in groups C and D than in group B,and lower in group D than in group C.The levels of CK,CK-MB and LDH of rats in groups B,C and D were higher than those in group A,while those in groups C and D were lower than those in group B,and group D was lower than group C.After intervention,the levels of IL-1β,IL-6 and TNF-αin groups B,C and D were higher than those in group A,while those in groups C and D were lower than those in group B,and those in group D were lower than those in group C.After intervention,the protein and mRNA expression levels of SIRT3 andβ-catenin in groups B,C and D were higher than those in group A,and those in groups C and D were higher than those in group B,and those in group D were higher than those in group C.At the same time,the protein expression and mRNA expression of PPARγin groups B,C

关 键 词：丹参多酚酸盐 心肌梗死 SIRT3/β-catenin-PPARγ 心肌损伤 心功能 

分 类 号：R-33[医药卫生] R542.22