微小核糖核酸-211和微小核糖核酸-196a在宫颈癌中的表达及意义  

The expression and significance of miR-211 and miR-196a in cervical cancer

作　　者：喻长法[1] 叶丽君[1] 蔡莎莎[1] 蒋亚萍[1] YU Chang-fa;YE Li-jun;CAI Sha-sha;JIANG Ya-ping(The First People Hospital of Taizhou,Taizhou,Zhejiang 318020,China)

机构地区：[1]台州市第一人民医院,浙江台州318020

出　　处：《中国妇幼保健》2023年第9期1692-1695,共4页Maternal and Child Health Care of China

基　　金：浙江省台州市科学技术局项目(21ywb53)。

摘　　要：目的探讨微小核糖核酸-211(miR-211)和微小核糖核酸-196a(miR-196a)在宫颈癌中的表达及意义。方法选取2017年1月—2020年6月在台州市第一人民医院妇科住院的宫颈癌患者79例为宫颈癌组,另选取同期来院30例健康体检者为对照组。采用实时荧光定量PCR检测外周血浆中的miR-211和miR-196a表达水平,并用SPSS 22.0软件进行统计学分析。结果宫颈癌组miR-211和miR-196a表达水平分别为(0.720±0.278)和(3.194±0.759),对照组miR-211和miR-196a达水平分别为(1.529±0.648)和(1.857±0.638),miR-211表达水平在宫颈癌组显著低于对照组,miR-196a表达水平在宫颈癌组显著高于对照组,差异均有统计学意义(t=9.148和-8.555,均P<0.05)。宫颈癌患者miR-211和miR-196a表达水平在临床分期、分化程度、淋巴结转移及HR-HPV感染方面差异均有统计学意义(均P<0.05)。miR-211对宫颈癌诊断的曲线下面积(AUC)为0.891,miR-196a对宫颈癌诊断的AUC为0.906,均有较高的诊断效能。结论miR-211可能为抑癌基因、miR-196a可能为癌基因,对宫颈癌具有较高的诊断效能。Objective To explore the expression and significance of miR-211 and miR-196a in cervical cancer.Methods A total of 79 patients with cervical cancer who were hospitalized in the gynecology department from January 2017 to June 2020 as the cervical cancer group,and 30 patients who came to the hospital for physical examination during the same period as the control group.Real-time fluorescent quantitative PCR was used to detect the expression levels of miR-211 and miR-196a in peripheral plasma.SPSS 22.0 software was used for statistical analysis.Results The expression levels of miR-211 and miR-196a in the cervical cancer group were(0.720±0.278)and(3.194±0.759),the expression levels of miR-211 and miR-196a in control group were(1.529±0.648)and(1.857±0.638).The expression level of miR-211 in the cervical cancer group was significantly lower than that in control group,while the expression level of miR-196a in the cervical cancer group was significantly higher than that in control group(t=9.148 and-8.555,P<0.05).The expression levels of miR-211 and miR-196a in cervical cancer patients were statistically significant in terms of clinical stage,degree of differentiation,lymph node metastasis and HR-HPV infection(P<0.05).The area under the curve(AUC)of miR-211 for cervical cancer diagnosis was 0.891,and the AUC of miR-196a for cervical cancer diagnosis was 0.906,both of which had high diagnostic efficiency.Conclusion miR-211 may be a tumor suppressor gene,and miR-196a may be an oncogene,which has high diagnostic efficiency for cervical cancer.

关 键 词：微小核糖核酸-211 微小核糖核酸-196a 宫颈癌 

分 类 号：R737.33[医药卫生—肿瘤]