circ_0000376靶向miR-876-3p调控结肠癌细胞进程  

circ_0000376 regulates colon cancer cell processes by targeting miR-876-3p

作　　者：熊隽禾 张力[2] 林圳滨 甘国莲 XIONG Jun-he;ZHANG Li;LIN Zhen-bin;GAN Guo-lian(The Second Clinical School of Guangzhou University of Traditional Chinese Medicine Guangzhou,Guangzhou,Guangdong Province 510400,China;Department of Proctology,Guangdong Province Traditional Chinese Medical Hospital,Guangzhou,Guangdong Province 510120,China)

机构地区：[1]广州中医药大学第二临床医学院,广东广州510400 [2]广东省中医院肛肠科,广东广州510120

出　　处：《解剖学研究》2023年第2期114-119,138,共7页Anatomy Research

摘　　要：目的 探讨circ_0000376对结肠癌细胞SW480迁移、侵袭和凋亡的影响及其分子机制。方法 选取30例结肠癌组织及其相应的癌旁组织;体外培养SW480细胞,根据实际转染蛋白情况分别设si-NC组、si-circ_0000376组、miR-NC组、miR-876-3p组、si-circ_0000376和anti-miR-876-3p共转染组以及sicirc_0000376和anti-miR-NC共转染组。采取RT-qPCR检测circ_0000376和miR-876-3p的表达水平、Transwell检测细胞迁移和侵袭、流式细胞术检测细胞凋亡、Western blot检测相关蛋白的表达、双荧光素酶报告基因实验检测circ_0000376和miR-876-3p的靶向关系。结果 结肠癌组织中circ_0000376较癌旁组织呈高表达(P<0.05),miR-876-3p较癌旁组织呈低表达(P<0.05);沉默circ_0000376或过表达miR-876-3p可有效降低癌细胞恶性行为活性,MMP-2、MMP-9、Bcl-2蛋白也呈更低表达,细胞凋亡活性Bax蛋白呈明显高表达(P<0.05);circ_0000376靶向调控miR-876-3p表达(P<0.05),并发现使miR-876-3p呈现低表达会造成circ_0000376对SW480细胞迁移、侵袭和凋亡形成逆转作用(P<0.05)。结论 沉默circ_0000376可通过靶向上调miR-876-3p抑制SW480细胞迁移和侵袭,并促进凋亡。Objective To investigate the effects of circ_0000376 on the migration,invasion and apoptosis of colon cancer cell line SW480 and its molecular mechanism.Methods Thirty colon cancer tissues and corresponding adjacent tissues were selected.SW480 cells were cultured in vitro and divided into si-NC group,si-circ_0000376 group,miR-NC group,miR-876-3p group,si-circ_0000376+anti-miR-NC group,si-circ_0000376+anti-miR-876-3p group.RT-qPCR was used to detect the expression levels of circ_0000376 and miR-876-3p.Transwell assay was used to detect cell migration and invasion.Flow cytometry was used to detect cell apoptosis.Western blot was used to detect the expression of related proteins.Dual luciferase reporter gene assay was used to detect the targeting relationship between circ_0000376 and miR-876-3p.Results Compared with adjacent tissues,the expression of circ_000376 and miR-876-3p in colon cancer tissues were increased and decreased,respectively(P<0.05).Silencing circ_0000376 or overexpressing miR-876-3p reduced the migration and invasion number of SW480 cells and the protein levels of MMP-2,MMP-9 and Bcl-2,and increased the apoptosis rate and Bax protein levels(P<0.05).circ_0000376 targeted regulation of miR-876-3p expression(P<0.05),and down-regulated miR-876-3p reversed the effects of circ_0000376 silencing on migration,invasion and apoptosis of SW480 cells(P<0.05).Conclusion Silencing circ_0000376 can inhibit the migration and invasion of SW480 cells and promote apoptosis through targeted up-regulation of miR-876-3p.

关 键 词：结肠癌 环状RNA circ_0000376 miR-876-3p SW480细胞株 

分 类 号：R735.35[医药卫生—肿瘤]