家畜早期妊娠诊断的研究进展  

Advance of Early Pregnancy Diagnosis in Livestock

作　　者：李雯乔 李发弟[1] 王新基 乐祥鹏[1] LI Wenqiao;LI Fadi;WANG Xinji;YUE Xiangpeng(Engineering Research Center of Grassland Agriculture,Ministry of Education,Key Laboratory of Grassland Livestock Innovation of Ministry of Agriculture and Rural Affairs,State Key Laboratory of Herbage Improvement and Grassland Agro-ecosystems,College of Pastoral Agriculture Science and Technology,Lanzhou University,Lanzhou 730020,China;Extension Station of Animal Husbandry and Veterinary Medicine in Minqin County,Minqin 733300,China)

机构地区：[1]兰州大学草地农业科技学院、草种创新与草地农业生态系统全国重点实验室、农业农村部草牧业创新重点实验室、草地农业教育部工程研究中心,兰州730020 [2]民勤县畜牧兽医工作站,民勤733300

出　　处：《畜牧兽医学报》2023年第5期1782-1791,共10页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：甘肃省科技重大专项计划(20ZD7NA004);甘肃省重点研发计划(20YF8NH158、21YF5NH213);国家肉羊产业技术体系(CARS-38)。

摘　　要：本文从原理、方法、诊断时间和准确率等方面对家畜妊娠临床诊断法、实验室诊断法以及胎儿游离DNA检测法进行综述,总结了不同妊娠诊断方法的优缺点、发展趋势以及实际应用效果,为探索不同应用场景下适宜的家畜妊娠诊断方法提供参考。传统的临床诊断法具有快捷经济的优点,但准确率受人为操作影响较大;实验室诊断法提高了准确率,提早了检测时间,但存在成本高和检测标准不统一的局限性;胎儿游离DNA检测法可用于家畜胎儿性别鉴定,而非性别依赖性胎儿DNA特异性标志物仍需进一步探索。This article reviewed the clinical diagnostics,laboratory diagnostics and circulating cell-free fetal DNA(ccffDNA)detection of pregnancy from the following four aspects:principle,method,diagnostic time and accuracy.Through the comparison and comprehensive analysis of the above methods,The advantages,limitations,research trends and the actual application effects of the above diagnostic methods were summarized,in order to provide suitable methods of livestock pregnancy diagnosis applied in different situations.The traditional clinical diagnostics have the double advantages of fast and cheap,but the accuracy is susceptible to the artificial operation.Laboratory diagnostics are earlier and more accurate,but they have disadvantages of lacking unified standard and costing more.The detection of ccffDNA can be used to identify fetal sex in domestic animals,whereas the specific markers of non-sex-dependent fetal DNA are still needed to be explored further.

关 键 词：放射免疫分析法 酶免疫测定法 早期妊娠因子 妊娠相关糖蛋白 胎儿游离DNA 

分 类 号：S814.8[农业科学—畜牧学]