基于脾脏转录组筛选北京油鸡和广明白鸡抗热应激相关功能基因  

Transcriptome Based Screening of Functional Genes Related to Heat Stress Resistance in Beijing You Chickens and Guangming Broilers

作　　者：王子渲 王巧[2] 张锦 Astrid Lissette Barreto Sanchez 郑麦青[2] 李庆贺[2] 崔焕先[2] 安炳星 赵桂苹[2] 文杰[2] 李和刚 WANG Zixuan;WANG Qiao;ZHANG Jin;Astrid Lissette Barreto Sanchez;ZHENG Maiqing;LI Qinghe;CUI Huanxian;AN Bingxing;ZHAO Guiping;WEN Jie;LI Hegang(College of Animal Science and Technology,Qingdao Agricultural University,Qingdao 266109,China;Institute of Animal Sciences,Chinese Academy of Agricultural Sciences,Beijing 100193,China)

机构地区：[1]青岛农业大学动物科技学院,青岛266109 [2]中国农业科学院北京畜牧兽医研究所,北京100193

出　　处：《畜牧兽医学报》2023年第5期1905-1914,共10页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家重点研发计划(2018YFE0128000);国家自然科学基金(32072708);国家现代农业产业技术体系建设专项(CARS-41);农业科技创新计划(ASTIP-IAS04,CAASZDRW202005)。

摘　　要：旨在通过对北京油鸡(BY)和“广明2号”白羽肉鸡B系(广明白鸡,GM)在热应激和正常饲养条件下的脾脏转录组进行分析,鉴定两鸡种在应对热应激时的差异表达基因和信号通路,解析不同鸡种耐热性能差异的分子调控机制。试验动物采用相同饲养条件下的25日龄北京油鸡和广明白鸡,测定异嗜性粒细胞与淋巴细胞的比值(H/L)、超氧化物歧化酶(SOD)和总抗氧化能力(T-AOC)等表型数据,采集脾脏组织进行转录组测序(RNA-Seq)。基于RNA-Seq数据,结合表型进行加权基因共表达网络分析(WGCNA),筛选出与性状相关性较高的模块与基因。脾脏转录组测序结果表明,热应激组相比于对照组,北京油鸡中共鉴定到313个差异表达基因,其中169个上调表达,144个下调表达;广明白鸡中鉴定到235个差异表达基因,其中152个上调表达,83个下调表达。利用WGCNA分析,在北京油鸡中筛选出2个与H/L相关性较高的模块,在广明白鸡中筛选出4个与H/L和T-AOC强相关的模块。通过筛选模块中的Hub基因,发现两品种中均存在TRIM 29基因,说明该基因可能在抗热应激方面发挥重要作用。本研究通过转录组分析,揭示了不同鸡种在热应激下基因的表达差异规律,并鉴定到与耐热相关的候选基因,为进一步研究鸡的耐热机制提供了新的思路和线索。The purpose of this study was to identify the differentially expressed genes(DEGs)and signaling pathways between Beijing You chicken and Guangming No.2 broiler line B(Guangming broiler)two chicken breeds in response to heat stress,and to reveal the molecular regulatory mechanisms underlying heat tolerance of different chicken breeds by analyzing spleen transcriptomic data of two chicken breeds Beijing You chicken and Guangming No.2 broiler line B(Guangming broiler)under heat stress and normal rearing conditions.The animals were 25-day-old Beijing You chickens and Guangming broilers under the same rearing managements.Heterophil to lymphocyte ratio(H/L),superoxide dismutase(SOD)and total antioxidant capacity(T-AOC)were measured,and spleen tissues were collected for RNA-Seq.WGCNA analysis was performed,in combination with gene expression matrix based on RNA-Seq data and the phenotypes,to screen the modules and genes with high correlation with the traits.Comparison of the transcriptome sequencing results between the control and heat stress groups showed that 313 DEGs in Beijing You chicken before and after heat stress,of which 169 genes were up-regulated and 144 genes were down-regulated;while 235 DEGs in Guangming broiler,of which 152 genes were up-regulated and 83 genes were down-regulated.Through WGCNA,2 modules with high correlation with H/L were screened in Beijing You chicken,while 4 modules with strong correlation with H/L and T-AOC were screened in Guangming broiler.Screening of the Hub gene in the module revealed the presence of the TRIM 29 gene in both breeds,which plays an important role under heat stress conditions.In this study,the differential patterns of gene expression in different chicken breeds under heat stress were revealed by RNA-Seq.The candidate genes related to heat tolerance were identified,which provided novel ideas and clues for further study of heat tolerance mechanism in chickens.

关 键 词：北京油鸡 广明白鸡 热应激 差异表达基因 加权基因共表达网络分析 

分 类 号：S831.2[农业科学—畜牧学]