利用GFP荧光标记比较大丽轮枝菌对抗/感马铃薯品种的侵染过程  

Utilization of GFP tagged Verticillium dahliae strain to evaluate dynamic infection on resistant/susceptible potato varieties

作　　者：康立茹 高婧 贾瑞芳[1] 刘燕 范俊臣 张之为[1] 赵君[1] KANG Liru;GAO Jing;JIA Ruifang;LIU Yan;FAN Junchen;ZHANG Zhiwei;ZHAO Jun(Inner Mongolia Agricultural University,College of horticulture and plant protection,Hohhot 010018,China;Inner Mongolia Academy of Agricultural&Animal Husbandry Sciences,Institute of Vegetables and Flowers,Hohhot 010031,China)

机构地区：[1]内蒙古农业大学,园艺与植物保护学院,呼和浩特010018 [2]内蒙古自治区农牧业科学院,蔬菜花卉研究所,呼和浩特010031

出　　处：《植物病理学报》2023年第2期164-172,共9页Acta Phytopathologica Sinica

基　　金：国家自然科学基金(31860495);内蒙古自然科学基金重点项目(2022ZD10);内蒙古科技攻关项目(2019GG180)。

摘　　要：为了对马铃薯黄萎病菌的系统侵染进行研究,本研究首先建立了用于观察大丽轮枝菌侵染马铃薯过程的培养皿滤纸体系,在此基础上利用带有GFP标记的大丽轮枝菌对不同抗性水平马铃薯根系侵染过程进行了比较研究,结果表明:马铃薯根系在浓度为107个·mL-1的大丽轮枝菌分生孢子液中浸泡处理2 h后,分生孢子可附着在抗/感马铃薯根系的根毛上,单个视野中感病品种根系上分生孢子附着平均数量为65个,显著高于抗病品种根系上附着的分生孢子数量(23个);接种12 hpi,根系上附着的分生孢子开始萌发,但抗/感品种根系表面孢子的萌发率无显著差异;接种3 dpi后,菌丝体能够突破根系表皮,进入细胞间隙,但感病品种根系中菌丝的扩展速度更快,并在7 d后到达根系的维管束内部;而抗病品种则在接种9 dpi,在维管束内才能够观察到绿色荧光信号;接种10 dpi,感病品种根系中可见新的分生孢子梗和分生孢子形成,而抗病品种中未见有分生孢子的形成。接种30 dpi,感病品种地上部茎杆中定殖的病原菌量是抗病品种的5倍以上;同时,感病品种叶柄中的病原菌数量也显著高于抗病品种。In order to study the systemic infection of Verticillium dahliae in potato,we firstly established a Petri dish filter paper system by which the evaluation of dynamic roots infection in resistant/susceptible potato varieties was conducted with the GFP fluorescence labelled Verticillium dahliae strain.The results showed that after soaking for 2 hours in a conidia suspension of 107conidia·mL-1,conidia could attach to the root hairs of resistant/susceptible potato roots,but a large amount of conidia was attached to the roots of susceptible varieties in which 65 conidia were on susceptible cultivar,while 23 conidia on resistant cultivar at average view of microscope.After 12 hours of inoculation,there was no significant difference in the germination rate of conidia on the roots surface of both resistant and susceptible varieties.Three days after inoculation,the mycelium could penetrate the root epidermis and enter the intercellular space of tissues.However,compared with the resistant varieties,the mycelium in the root system of the susceptible varieties grew faster and reached the inner vascular bundle of the root system at 7 dpi,and the green fluorescence signal was observed in the vascular bundle of resistant varieties only at 9 dpi.At 10 dpi,new conidia stalk and conidia formation could be seen in the roots of susceptible varieties,while no conidia formation in the resistant varieties.The amount of pathogen colonization in the aerial stems of susceptible potato varieties at 30 dpi was five times more than that of the resistant varieties.At the same time,the number of pathogens in the petioles of susceptible varieties was also significantly higher than that of resistant varieties.

关 键 词：马铃薯 大丽轮枝菌 侵染过程 抗性比较 

分 类 号：S432.44[农业科学—植物病理学]