The extraction of effective components and an antioxidant activity study of Tulipa edulis  

作　　者：Doudou Zhang Dong Xiao Tingting Yin Shuangzhi Zhao Olena Zhur Xun Xiao Hailun He Leilei Chen 

机构地区：[1]Beijing Advanced Innovation Center for Food Nutrition and Human Health,Beijing Technology and Business University,Beijing 102488,China [2]School of Life Sciences,Central South University,Changsha 410013,China [3]State Key Laboratory of Coal Resources and Safe Mining,University of Mining and Technology,Xuzhou 221116,China [4]Institute of Agro-Food Science and Technology,Shandong Academy of Agricultural Sciences,Jinan 250100,China

出　　处：《Food Science and Human Wellness》2024年第1期276-286,共11页食品科学与人类健康（英文）

基　　金：supported by the Open Grant of Beijing Advanced Innovation Center for Food Nutrition and Human Health(20182024);National Natural Science Foundation of China(31370104);The Natural Science Foundation of Hunan Province,China(2021JJ30029);the Taishan Scholar Program of Shandong Province,China(tsqn201909168);“Double Hundred”Program for Foreign Experts of Shandong Province,China(WST2017004);Hunan Province Postgraduate Education Innovation Project and Professional Capacity Enhancement(CX20200297);Project the Fundamental Research Funds for the Central Universities of Central South University(2020zzts424,2020zzts422)。

摘　　要：Plant extracts from natural sources are an excellent choice for food additives and natural antioxidants.In this study,the active components of Tulipa edulis were extracted and analysed,and their antioxidant capacity was measured.Then,the crude extract mixture was separated and purified using a Sephadex LH-20 gel,and the antioxidant activity of the purified products was determined.Human umbilical vein endothelial human umbilical vein endothelial cells(HUVEC)cells were treated with 35 mmol/L glucose to construct a model of oxidative stress.Then,the cells were treated with the active component to observe whether the products of T.edulis could have a good protective effect on HUVEC cells induced by glucose.Transcriptome analysis was also performed on HUVEC cells after same treatment to explore the possible mechanism of the component F2 protecting HUVEC cells from oxidative stress induced by high glucose.The results showed that component F2 obtained from T.edulis has strong antioxidant activity.Moreover,F2 can play a strong antioxidant protective role in HUVEC cells.Meanwhile,the gene expression of heme oxygenase 1(HO-1),γ-glutamyl cysteine ligase catalytic subunit(GCLC)and NAD(P)H quinone oxidoreductase-1(NQO1)in HUVEC cells was up-regulated after treated with F2.This study provides reference value for the further development and application of T.edulis and the d evelopment of functional food.

关 键 词：Tulipa edulis Oxidative stress PURIFICATION ANTIOXIDANT 

分 类 号：TS202.3[轻工技术与工程—食品科学]