Mogroside IIE,an in vivo metabolite of sweet agent,alleviates acute lung injury via Pla2g2a-EGFR inhibition  

作　　者：Weichao Lü Guoqing Ren Kuniyoshi Shimizu Renshi Li Chaofeng Zhang 

机构地区：[1]State Key Laboratory of Natural Medicines,School of Traditional Chinese Pharmacy,China Pharmaceutical University,Nanjing 211198,China [2]Sino-Jan Joint Lab of Natural Health Products Research,School of Traditional Chinese Pharmacy,China Pharmaceutical University,Nanjing 211198,China [3]Faculty of Agriculture,Kyushu University,Fukuoka 819-0395,Japan

出　　处：《Food Science and Human Wellness》2024年第1期299-312,共14页食品科学与人类健康（英文）

基　　金：the National Natural Science Foundation(81773982,82003937);Youth Academic leaders of the Qinglan Project in Jiangsu province for financial support。

摘　　要：In the face of increasingly serious environmental pollution,the health of human lung tissues is also facing serious threats.Mogroside IIE(M2E)is the main metabolite of sweetening agents mogrosides from the anti-tussive Chinese herbal Siraitia grosvenori.The study elucidated the anti-inflammatory action and molecular mechanism of M2E against acute lung injury(ALI).A lipopolysaccharide(LPS)-induced ALI model was established in mice and MH-S cells were employed to explore the protective mechanism of M2E through the western blotting,co-immunoprecipitation,and quantitative real time-PCR analysis.The results indicated that M2E alleviated LPS-induced lung injury through restraining the activation of secreted phospholipase A2 type IIA(Pla2g2a)-epidermal growth factor receptor(EGFR).The interaction of Pla2g2a and EGFR was identified by co-immunoprecipitation.In addition,M2E protected ALI induced with LPS against inflammatory and damage which were significantly dependent upon the downregulation of AKT and m TOR via the inhibition of Pla2g2a-EGFR.Pla2g2a may represent a potential target for M2E in the improvement of LPS-induced lung injury,which may represent a promising strategy to treat ALI.

关 键 词：Mogroside IIE Acute lung injury Secreted phospholipase A2 type IIA(Pla2g2a) Epidermal growth factor receptor(EGFR) 

分 类 号：TS201.4[轻工技术与工程—食品科学]