Evaluation of the intracellular lipid-lowering effect of polyphenols extract from highland barley in HepG2 cells  

作　　者：Yijun Yao Zhifang Li Bowen Qin Xingrong Ju Lifeng Wang 

机构地区：[1]College of Food Science and Engineering/Collaborative Innovation Centre for Modern Grain Circulation and Safety,Nanjing University of Finance and Economics,Nanjing 210023,China

出　　处：《Food Science and Human Wellness》2024年第1期454-461,共8页食品科学与人类健康（英文）

基　　金：financially supported by the National Key Research and Development Program of China(2021YFD2100904);the National Natural Science Foundation of China(31871729,32172147);the Modern Agriculture key Project of Jiangsu Province of China(BE2022317);the Modern Agricultural Industrial Technology System Construction Project of Jiangsu Province of China(JATS[2021]522);a project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)。

摘　　要：Active ingredients from highland barley have received considerable attention as natural products for developing treatments and dietary supplements against obesity.In practical application,the research of food combinations is more significant than a specific food component.This study investigated the lipid-lowering effect of highland barley polyphenols via lipase assay in vitro and HepG2 cells induced by oleic acid(OA).Five indexes,triglyceride(TG),total cholesterol(T-CHO),low density lipoprotein-cholesterol(LDL-C),aspartate aminotransferase(AST),and alanine aminotransferase(ALT),were used to evaluate the lipidlowering effect of highland barley extract.We also preliminary studied the lipid-lowering mechanism by Realtime fluorescent quantitative polymerase chain reaction(q PCR).The results indicated that highland barley extract contains many components with lipid-lowering effects,such as hyperoside and scoparone.In vitro,the lipase assay showed an 18.4%lipase inhibition rate when the additive contents of highland barley extract were 100μg/m L.The intracellular lipid-lowering effect of highland barley extract was examined using 0.25 mmol/L OA-induced HepG2 cells.The results showed that intracellular TG,LDL-C,and T-CHO content decreased by 34.4%,51.2%,and 18.4%,respectively.ALT and AST decreased by 51.6%and 20.7%compared with the untreated hyperlipidemic HepG2 cells.q PCR results showed that highland barley polyphenols could up-regulation the expression of lipid metabolism-related genes such as PPARγand Fabp4.

关 键 词：Highland barley Polyphenols extract Lipid-lowering effect HepG2 cells 

分 类 号：TS201.4[轻工技术与工程—食品科学]