粗毛纤孔菌三萜成分分析及其对HepG 2细胞模型的脂质调节作用  

Component analysis of Inonotus hispidus triterpenes and their regulation on lipid content in HepG 2 cell model

作　　者：李德海[1,2] 刘东超 刘爽爽 霍永红 王占斌 LI Dehai;LIU Dongchao;LIU Shuangshuang;HUO Yonghong;WANG Zhanbin(College of Forestry,Northeast Forest University,Harbin 150040,Heilongjiang,China;Key Laboratory of Forest Food Resources Utilization of Heilongjiang Province,Harbin 150040,Heilongjiang,China)

机构地区：[1]东北林业大学林学院,黑龙江哈尔滨150040 [2]黑龙江省森林食品资源利用重点实验室,黑龙江哈尔滨150040

出　　处：《陕西师范大学学报（自然科学版）》2023年第3期65-74,共10页Journal of Shaanxi Normal University：Natural Science Edition

基　　金：黑龙江省自然科学基金(LH2022C007,C2015062);哈尔滨市科技局创新研究基金(2017RAQXJ091)。

摘　　要：为探索粗毛纤孔菌中的总三萜活性成分及其体外降脂能力,采用超高液相色谱-飞行时间质谱联用技术(UPLC-TOF-MS/MS)对粗毛纤孔菌中的总三萜进行定性分析,并通过油酸诱导建立HepG_(2)细胞高脂模型,以HepG_(2)细胞高脂模型的存活率、脂质积累量和血脂四项为指标,考察粗毛纤孔菌纯化后总三萜的体外降脂能力。根据UPLC-TOF-MS/MS得到的洗脱顺序、保留时间、分子量、质谱信息,结合文献,共鉴定出粗毛纤孔菌中三萜成分8种。体外实验表明,400μmol/L油酸孵育HepG_(2)细胞24 h时,HepG_(2)细胞内的脂质积累量最高。相较于其他浓度组,总三萜质量浓度为250μg/mL时,高脂HepG_(2)细胞内的脂质含量下降最为显著,总胆固醇、甘油三酯、低密度脂蛋白胆固醇的清除率分别达到41.86%、44.44%、44.02%,高密度脂蛋白胆固醇的增长率达到57.33%。研究结果证实了粗毛纤孔菌三萜的体外降脂功能,为开发新型降血脂功能食品提供了实验依据。In order to explore the active constituents of total triterpenoids from Inonotus hispidus and their ability to reduce lipid in vitro,ultrahigh liquid phase tandem high resolution mass spectrometry(UPLC-TOF-MS/MS)was used to qualitatively analyze the total triterpenoids,and the HepG_(2) cell high lipid model was established by induction of oleic acid.The survival rate and lipid content of the established HepG_(2) cell model were taken as indicators to investigate the lipid-lowering ability of total triterpenoids purified from Inonotus hispidus in vitro.The results showed that a total of 8 triterpenes were identified by UPLC-TOF-MS/MS based on the elution sequence,retention time,molecular weight,mass spectrometry and information referred to literature.Experiments in vitro showed that HepG_(2) cells incubated with 400μmol/L oleic acid for 24 h had the highest lipid accumulation amount.Compared with other concentration groups,the decrease in lipid content was most significant when the total triterpenoids was 250μg/mL,the total cholesterol,triglycerides and low density lipoprotein cholesterol contents decreased 41.86%,44.44%,44.02%,respectively,while the high density lipoprotein cholesterol content increased 57.33%in contrast to the control group.Our finding confirms the lipid-lowering function of triterpenes from Inonotus hispidus in vitro,and provides experimental basis for the development of new lipid-lowering functional foods.

关 键 词：粗毛纤孔菌 三萜 UPLC-TOF-MS/MS 体外降脂 HepG 2细胞高脂模型 

分 类 号：S567.3[农业科学—中草药栽培]