肉桂对肾阳虚大鼠下丘脑-垂体-靶腺轴相关mRNA表达及组织病理变化的影响  

Effects of Rougui(Cinnamomi Cortex)on Expression of Related mRNA and Histopathological Changes of Hypothalamus-Pituitary-Target Gland Axis in Rats with Kidney Yang Deficiency

作　　者：张哲 赵雯雯 孙秀蕊 闫天月 张玉杰[1] ZHANG Zhe;ZHAO Wenwen;SUN Xiurui;YAN Tianyue;ZHANG Yujie(School of Chinese Materia Medica,Bejing University of Chinese Medicine,Beijing 102488,China)

机构地区：[1]北京中医药大学中药学院,北京102488

出　　处：《中华中医药学刊》2023年第4期59-63,I0016,共6页Chinese Archives of Traditional Chinese Medicine

基　　金：国家自然科学基金面上项目(81673680)。

摘　　要：为探讨肉桂补肾助阳的作用机制,对连续灌胃肉桂后的肾阳虚大鼠下丘脑-垂体-靶腺轴相关mRNA表达及组织病理变化进行了考察。采用腹腔注射氢化可的松复制肾阳虚证大鼠模型,将大鼠分为对照组(CON组)、肾阳虚模型组(MOD组)、低剂量肉桂组(RGL组)和高剂量肉桂组(RGH组)。RGH组和RGL组大鼠分别连续4周灌胃不同剂量的肉桂提取液(8.1 g·kg^(-1)·d^(-1)和4.05 g·kg^(-1)·d^(-1)),CON组和MOD组给予相同体积生理盐水。实验结束后,分别检测血液生化指标和下丘脑-垂体-靶腺轴相关腺体脏器指数、血浆激素水平、mRNA表达,及相关组织的病理变化。结果显示,与CON组比,MOD组下丘脑指数、肾上腺指数、胸腺指数、甲状腺指数和睾丸指数均显著降低(P<0.01),垂体指数有降低趋势但无显著性差异;大鼠血浆促肾上腺激素(ACTH)、皮质酮(CORT)、三碘甲状腺原氨酸(T3)、四碘甲状腺原氨酸(T4)、睾酮(T)水平和下丘脑促甲状腺激素释放激素(TRH)mRNA、促性腺激素释放激素(Gn-RH)mRNA表达水平均显著降低(P<0.05),血浆雌二醇(E2)水平下丘脑促肾上腺激素释放激素(CRH)mRNA表达水平均显著升高(P<0.05),下丘脑、肾上腺、胸腺、睾丸病理组织形态也发生病理改变;与MOD组相比,RGL组和RGH组各项指标均显著降低(P>0.05),病理损伤有所减轻。实验验证了肉桂对肾阳虚大鼠的改善作用,其机制与下丘脑-垂体-靶腺轴的全面调控作用相关。研究可为揭示肉桂补肾作用机制,为肾阳虚证的临床防治奠定基础。In order to study the mechanism of tonifying kidney and strengthening Yang by Rougui(Cinnamomi Cortex),the expression of hypothalamic-pituitary-target gland related mRNA and histopathological changes of kidney-Yang deficiency rats were investigated.The rats were divided into normal group(CON),kidney-Yang deficiency model group(MOD),low-dose Rougui(Cinnamomi Cortex)group(RGL)and high-dose Rougui(Cinnamomi Cortex)group(RGH).Rats in RGH group and RGL group were given different doses of Rougui(Cinnamomi Cortex)extract(8.1 g·kg^(-1)·d^(-1)and 4.05 g·kg^(-1)·d^(-1))for 4 weeks,respectively.Rats in CON group and MOD group were given the same volume of normal saline.At the end of the experiment,the levels of serum biochemistry,hypothalamic-pituitary-target gland related organ index,plasma hormone,mRNA expression and the pathological changes of related tissues were measured.The results showed that the hypothalamus index,adrenal index,thymus index,thyroid index and testis index in MOD group were significantly lower than those in CON group(P<0.01).The levels of adrenocorticotropic hormone(ACTH),corticosterone(CORT),triiodothyronine(T3),tetraiodothyronine(T4),testosterone(T)and hypothalamic thyrotropin releasing hormone(TRH)mRNA,gonadotropin releasing hormone(GnRH)were significantly decreased(P<0.05).The level of plasma estradiol(E2)and hypothalamic corticotropin-releasing hormone(CRH)mRNA expressions were significantly increased(P<0.05).The pathological changes of hypothalamus,adrenal gland,thymus and testis were also observed.Compared with the MOD group,RGL group and RGH group showed significant regression(P>0.05).The pathological damage has also abated.In this experiment,the regulation of Rougui(Cinnamomi Cortex)on kidney-Yang deficiency syndrome is related to the regulation of hypothalamus-pituitary-target gland axis.This study may reveal the mechanism of Rougui(Cinnamomi Cortex)tonifying kidney and lay a foundation for clinical prevention and treatment of kidney-yang deficiency syndrome.

关 键 词：肉桂 肾阳虚证 下丘脑-垂体-靶腺轴 MRNA表达 组织病理 

分 类 号：R282.71[医药卫生—中药学]