一株高效反硝化脱氮菌的分离鉴定与脱氮性能  

Screening and Denitrification Performance of a Highly Efficient Denitrifying Bacterial Strain

作　　者：孙兆博 严滨 叶茜 陈小夫 张亚玲 SUN Zhaobo;YAN Bin;YE Qian;CHEN Xiaofu;ZHANG Yaling(School of Environmental Science&Engineering,Xiamen University of Technology,Xiamen 361024,China;Key Laboratory of Membrane Technology R&D and Application,Xiamen,361024 China)

机构地区：[1]厦门理工学院环境科学与工程学院,福建厦门361024 [2]厦门市膜技术研发与应用重点实验室,福建厦门361024

出　　处：《厦门理工学院学报》2023年第1期90-96,共7页Journal of Xiamen University of Technology

基　　金：科技小巨人认定资助项目(2020LYF6007)。

摘　　要：从厦门市某污水处理厂的活性污泥中分离纯化得到一株具有高效脱氮能力的菌株ZB-1,通过菌落形态、生理生化和16S rDNA序列分析,初步鉴定为斯氏假单胞菌(Pseudomonas stutzeri)。对菌株ZB-1在不同初始pH值、菌液接种量、碳源及NO3--N浓度条件下脱氮性能的分析结果表明,最优条件为pH7~8、丁二酸钠为碳源、接种量为3%,培养48 h后,其脱氮率为90.81%;菌株ZB-1具有较强的耐受性,对高浓度(200 mg·L^(-1)和500 mg·L^(-1))的NO3--N废水48 h脱氮率分别为70.61%和28.00%,7 d内可完全去除,表明菌株ZB-1在处理高浓度NO3--N废水的实际应用中具有一定的优势。A strain ZB-1 with high nitrogen removal ability was isolated and purified from the activated sludge of a sewage treatment plant in Xiamen,China.Through colony morphology,physiological and biochemical analysis,and 16S rDNA sequence analysis,it was preliminarily identified as Pseudomonas stutzeri.The analysis of nitrogen removal performance of strain ZB-1 under different initial pH,inoculum amount,carbon source,and nitrate nitrogen concentration conditions showed that the nitrogen removal rate was 90.81%after 48 hours of incubation with pH=7.2,sodium succinate as carbon source,and inoculum amount of 3%.The strain ZB-1 has a strong tolerance,with a nitrogen removal rate of 70.61%and 28.00%for high concentration(200 mg·L^(-1) and 500 mg·L^(-1))nitrate nitrogen wastewater within 48 hours,respectively,and can be completely removed within 7 days,indicating that the strain ZB-1 has a certain advantage in the practical application of treating high concentration nitrate nitrogen wastewater.

关 键 词：反硝化脱氮菌 筛选鉴定 脱氮性能 斯氏假单胞菌 

分 类 号：X172[环境科学与工程—环境科学]