miRNA-27与宫颈癌细胞线粒体凋亡途径的相关性  

Correlation between miRNA-27 and mitochondrial apoptosis pathway in cervical cancer cells

作　　者：王茜 雷正瑶 王顺利 莫琼华 易祥华[2,3] 蒋铭华 WANG Qian;LEI Zhengyao;WANG Shunli;MO Qionghua;YI Xianghua;JIANG Minghua(Department of Pathology,Tinglin Hospital,Jinshan District,Shanghai 201505,China;Department of Pathology,Tongji Hospital of Tongji University,Shanghai 200065,China;Xinjiang Key Laboratory of Clinical Genetic Testing and Biomedical Information,Karamay 834000,Xinjiang,China;Department of Gynaecology and Obstetrics,Minhang Hospital Affiliated to Fudan University/Central Hospital of Minhang District,Shanghai 201100,China)

机构地区：[1]上海市金山区亭林医院病理科,上海201505 [2]同济大学附属同济医院病理科,上海200065 [3]新疆临床基因检测与生物医学信息重点实验室,新疆克拉玛依834000 [4]复旦大学附属闵行医院/上海市闵行区中心医院妇产科,上海2011000

出　　处：《癌症进展》2023年第7期758-762,798,共6页Oncology Progress

基　　金：国家自然科学基金(82260322);上海市科学技术委员会科研计划项目(19ZR1448500、19411964700);上海市卫生局科研课题(20134034);上海市金山区卫生健康委员会科研课题(JSKJ-KTMS-2019-22)。

摘　　要：目的探讨微小RNA(miRNA)-27与宫颈癌细胞线粒体凋亡途径的相关性。方法通过miRNA数据库对miRNA-27进行生物信息学分析,并在体外构建宫颈癌细胞SiHa凋亡模型,采用实时荧光定量聚合酶链反应检测相关分子表达改变。结果miRNA-27存在4种成熟体hsa-miRNA-27a-3p、hsa-miRNA-27a-5p、hsa-miRNA-27b-3p和hsa-miRNA-27b-5p,在宫颈癌组织中表达不一,其靶基因参与多种生物学功能和信号通路,并与线粒体凋亡途径密切相关,其中靶基因LETM1结构域蛋白1(LETMD1)与宫颈癌密切相关并与miRNA-27共表达存在相关性,进一步在宫颈癌细胞SiHa凋亡模型中验证发现,hsa-miRNA-27a-3p、hsa-miRNA-27a-5p表达下调而LETMD1表达升高。结论miRNA-27与宫颈癌细胞线粒体凋亡途径密切相关,可能与LETMD1相关,有望成为潜在的治疗靶点。Objective To investigate the correlation between microRNA(miRNA)-27 and mitochondrial apoptosis pathway in cervical cancer cells.Method The bioinformatics analysis of miRNA-27 was carried out through the miRNA database,the SiHa apoptosis model of cervical cancer cells were constructed in vitro,and the expression changes of related molecules were detected by real-time fluorescent quantitative polymerase chain reaction.Result There were four mature forms of miRNA-27,hsa-miRNA-27a-3p,hsa-miRNA-27a-5p,hsa-miRNA-27b-3p,and hsa-miRNA-27b-5p.They were expressed differently in cervical cancer tissues,and their target genes were involved in various biological functions and signaling pathways,and closely related to the mitochondrial apoptosis pathway.Among them,the target gene LETM1 domain containing 1(LETMD1)was closely related to cervical cancer and correlated with the co-expression of miRNA-27.Further verification in the cervical cancer cell SiHa apoptosis model found that the expression of hsa-miRNA-27a-3p and hsa-miRNA-27a-5p were down-regulated while the expression of LETMD1 was up-regulated.Conclusion miRNA-27 is closely related to the mitochondrial apoptosis pathway of cervical cancer cells and may be related to LETMD1,which is expected to become a promising therapeutic target.

关 键 词：宫颈癌 微小RNA 凋亡 线粒体 生物信息学 

分 类 号：R737.33[医药卫生—肿瘤]