Label-free impedimetric immunosensor for point-of-care detection of COVID-19 antibodies  

作　　者：Lian C.T.Shoute Gaser N.Abdelrasoul Yuhao Ma Pedro A.Duarte Cole Edwards Ran Zhuo Jie Zeng Yiwei Feng Carmen L.Charlton Jamil N.Kanji Shawn Babiuk Jie Chen 

机构地区：[1]Department of Electrical and Computer Engineering,University of Alberta,Edmonton,AB,T6G 2V4,Canada [2]Public Health Laboratory,Alberta Precision Laboratories,Edmonton,AB,Canada [3]Department of Laboratory Medicine and Pathology,University of Alberta,Edmonton,AB,T6G 2B7,Canada [4]Li Ka Shing Institute for Virology,University of Alberta,Edmonton,AB,Canada [5]Division of Infectious Diseases,Department of Medicine,Cumming School of Medicine,University of Calgary,Calgary,AB,Canada [6]Department of Pathology&Laboratory Medicine,Cumming School of Medicine,University of Calgary,Calgary,AB,Canada [7]National Centre for Foreign Animal Disease,Canadian Food Inspection Agency,Winnipeg,MB,Canada [8]Department of Immunology,University of Manitoba,Winnipeg,MB,Canada [9]Department of Biomedical Engineering,University of Alberta,Edmonton,AB,T6G 2R3,Canada

出　　处：《Microsystems & Nanoengineering》2023年第1期161-176,共16页微系统与纳米工程（英文）

摘　　要：The COVID-19 pandemic has posed enormous challenges for existing diagnostic tools to detect and monitor pathogens.Therefore,there is a need to develop point-of-care(POC)devices to perform fast,accurate,and accessible diagnostic methods to detect infections and monitor immune responses.Devices most amenable to miniaturization and suitable for POC applications are biosensors based on electrochemical detection.We have developed an impedimetric immunosensor based on an interdigitated microelectrode array(IMA)to detect and monitor SARS-CoV-2 antibodies in human serum.Conjugation chemistry was applied to functionalize and covalently immobilize the spike protein(S-protein)of SARS-CoV-2 on the surface of the IMA to serve as the recognition layer and specifically bind anti-spike antibodies.Antibodies bound to the S-proteins in the recognition layer result in an increase in capacitance and a consequent change in the impedance of the system.The impedimetric immunosensor is label-free and uses non-Faradaic impedance with low nonperturbing AC voltage for detection.The sensitivity of a capacitive immunosensor can be enhanced by simply tuning the ionic strength of the sample solution.The device exhibits an LOD of 0.4 BAU/ml,as determined from the standard curve using WHO IS for anti-SARS-CoV-2 immunoglobulins;this LOD is similar to the corresponding LODs reported for all validated and established commercial assays,which range from 0.41 to 4.81 BAU/ml.The proof-of-concept biosensor has been demonstrated to detect anti-spike antibodies in sera from patients infected with COVID-19 within 1 h.

关 键 词：ANTIBODIES IMPEDANCE free 

分 类 号：R563.1[医药卫生—呼吸系统] TP212[医药卫生—内科学]