Inhibition of H_(2)O_(2)-induced TM3 cell apoptosis by oxidative stress by lentinan functionalized selenium nanoparticles through JAK2/STAT-3 and P53 pathways  

作　　者：MIAOMIAO LI ZILIN ZHENG JUNYI KE JIEYI LUO FAN JIANG YANXIA QU BING ZHU YINGHUA LI LIANDONG ZUO 

机构地区：[1]Department of Andrology,Guangzhou Women and Children’s Medical Center,Guangzhou Medical University,Guangzhou,510120,China [2]Center Laboratory,Guangzhou Women and Children’s Medical Center,Guangzhou Medical University,Guangzhou,510120,China

出　　处：《BIOCELL》2023年第6期1397-1405,共9页生物细胞（英文）

基　　金：Science and Technology Projects in Guangzhou(202201020632,202201020638,202206010100,202201020655,and 202102010202);the Guangdong Natural Science Foundation(2020A1515110648);the Open Project of Guangdong Key Laboratory of Marine Materia(LMM2020-7);the Open Fund of Guangdong Provincial Key Laboratory of Functional Supramolecular Coordination Materials and Applications(2020A03);the Guangzhou Medical University Students’Science and Technology Innovation Project(2021AEK119 and 02-408-2203-2079).

摘　　要：Background:Nano-selenium has been widely used in antiviral and anticancer therapy,and has the advantages of good targeting and low toxicity.For the first time,we combined male reproduction with nano-selenium to investigate its antioxidant effect.This study investigated the protective effect of lentinan functionalized selenium nanoparticles on oxidative stress injury of the hydrogen peroxide(H_(2)O_(2))-induced Leydig cell line,TM3.Methods:The suitable concentration of nano-selenium treatment to promote cell proliferation was also discussed.The concentration of 4μM could significantly promote the growth of TM3 cells.Oxidative stress damage was caused using an 800μM concentration of hydrogen peroxide.The cells were divided into four groups:normal control group,oxidative stress treatment group,H_(2)O_(2)+SeNPs@LNT group,and SeNPs@LNT group.The H_(2)O_(2)+SeNPs@LNT group was pretreated with 4μM of SeNPs@LNT for 12 h,followed by 800μM of H_(2)O_(2)for 8 h.Results:Nano-selenium could significantly promote the proliferation and viability of TM3 cells.SeNPs@LNT treatment increased the level of mitochondrial membrane potential in normal cells and slowed down the decline in mitochondrial membrane potential level caused by oxidative stress injury.In addition,the increase in reactive oxygen species caused by oxidative stress was inhibited by SeNPs@LNT treatment.The apoptosis of TM3 cells was detected,and SeNPs@LNT alleviated the necrosis and apoptosis of TM3 cells induced by H_(2)O_(2).Nano-selenium plays a protective role against oxidative H_(2)O_(2)-induced stress injury in TM3 cells through the changes in the Janus kinase 2/signal transducer and activator of transcription 3 signaling pathway and P53 pathway,and the expression levels of other related proteins,protein kinase B(AKT)and C3.Conclusion:SeNPs@LNT exhibited good biological activity and antioxidant effect and can thus be used to protect the male reproductive system from oxidative stress.

关 键 词：Selenium nanoparticles LENTINAN ROS Antioxidant 

分 类 号：R96[医药卫生—药理学]