茶多酚对脂多糖刺激下成骨细胞增殖分化的影响  

Effect of tea polyphenols on proliferation and differentiation of osteoblasts stimulated by lipopolysaccharide

作　　者：秦祎雄 袁子健 谢山周 祝英文 陈明慧 韩标 郭勇[1] Qin Yixiong;Yuan Zijian;Xie Shanzhou;Zhu Yingwen;Chen Minghui;Han Biao;Guo Yong(Guangxi Higher Education Key Laboratory of Biochemistry and Molecular Biology,College of Intelligent Medicine and Biotechnology,Guilin Medical University,Guilin 541199,Guangxi Zhuang Autonomous Region,China)

机构地区：[1]桂林医学院智能医学与生物技术学院,广西高校生物化学与分子生物学重点实验室,广西壮族自治区桂林市541199

出　　处：《中国组织工程研究》2023年第35期5665-5669,共5页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金项目(32071309),项目负责人:郭勇;广西壮族自治区自然科学基金项目(2018GXNSFAA281357),项目负责人:郭勇;国家级大学生创新创业项目(202010601027),项目负责人:陈明慧。

摘　　要：背景:研究表明,茶多酚在牙周炎等疾病的研究中能发挥抗炎、抗氧化作用。目的:探讨茶多酚在脂多糖刺激下对小鼠成骨细胞增殖分化及氧化应激的影响。方法:体外培养小鼠成骨细胞MC3T3-E1,分4组处理:对照组常规培养;脂多糖组加入10μg/mL脂多糖,茶多酚组加入1μg/mL茶多酚,联合组加入10μg/mL脂多糖+1μg/mL茶多酚。采用MTT法检测细胞增殖情况,碱性磷酸酶试剂盒检测细胞碱性磷酸酶活性,偶氮偶联法检测细胞碱性磷酸酶染色程度,钙检测试剂盒检测细胞钙沉积量,超氧化物试剂盒检测细胞超氧化物含量,丙二醛试剂盒检测细胞丙二醛含量。结果与结论:①与对照组相比,脂多糖组成骨细胞增殖活性、碱性磷酸酶活性、钙沉积量降低(P<0.05),碱性磷酸酶染色程度降低(P<0.01),超氧化物和丙二醛含量增加(P<0.05);茶多酚组成骨细胞增殖活性、碱性磷酸酶活性、钙沉积量升高(P<0.05),碱性磷酸酶染色程度提高(P<0.01),超氧化物和丙二醛含量减少(P<0.05);②与脂多糖组相比,联合组成骨细胞增殖活性、钙沉积量升高(P<0.05),碱性磷酸酶染色程度提高(P<0.001),超氧化物和丙二醛含量减少(P<0.05);③结果表明,茶多酚可以促进成骨细胞的增殖分化,降低氧化应激因子的表达水平,逆转脂多糖对成骨细胞的不良影响。BACKGROUND:Studies have shown that tea polyphenols can play anti-inflammatory and antioxidant effects in the investigation of periodontitis and other diseases.OBJECTIVE:To investigate the effects of tea polyphenols on the proliferation,differentiation and oxidative stress of mouse osteoblasts induced by lipopolysaccharide.METHODS:The mouse osteoblasts MC3T3-E1 were cultured in vitro,and the MC3T3-E1 cells were randomly divided into four groups,namely the control group(conventional culture),the lipopolysaccharide group(10μg/mL lipopolysaccharide),the tea polyphenols group(1μg/mL tea polyphenols),and the combination group(10μg/mL lipopolysaccharide+1μg/mL tea polyphenols).The MTT assay was used to detect the cell proliferation.The alkaline phosphatase activity was detected by alkaline phosphatase kit.The alkaline phosphatase staining degree was detected by alkaline phosphatase azo coupling method.The calcium deposition amount of the cells was detected by calcium detection kit.The superoxide content of cells was detected by superoxide kit.The malondialdehyde content of cells was detected by malondialdehyde kit.RESULTS AND CONCLUSION:(1)Compared with the control group,the proliferation activity,alkaline phosphatase activity and calcium deposition of osteoblasts were significantly decreased in the lipopolysaccharide group(P<0.05);the staining degree of alkaline phosphatase was significantly decreased(P<0.01);and the contents of superoxide and malondialdehyde were significantly increased(P<0.05).The proliferation activity,alkaline phosphatase activity and calcium deposition of osteocytes in the tea polyphenols group were significantly increased(P<0.05);the staining degree of alkaline phosphatase was significantly increased(P<0.01);and the contents of superoxide and malondialdehyde were significantly decreased(P<0.05).(2)Compared with the lipopolysaccharide group,the proliferation activity and calcium deposition of bone cells in the combination group were significantly increased(P<0.05);the staining degree of alkali

关 键 词：成骨细胞 脂多糖 茶多酚 增殖 成骨分化 氧化应激 

分 类 号：R459.9[医药卫生—治疗学] R34[医药卫生—临床医学] R285.5