冷藏期内红阳猕猴桃软腐病病原菌的分离与鉴定  

Isolation and Identification of Pathogenic Fungi Causing Fruit Rot of Hongyang Kiwifruit during Cold Storage

作　　者：凌立贞 陈林燕 涂丹丹 张书东 LING Lizhen;CHEN Linyan;TU Dandan;ZHANG Shudong(School of Biological Science and Technology,Liupanshui Normal University,Liupanshui Guizhou 553004)

机构地区：[1]六盘水师范学院生物科学与技术学院,贵州六盘水553004

出　　处：《安徽农学通报》2023年第6期114-118,共5页Anhui Agricultural Science Bulletin

基　　金：贵州省科学技术基金资助项目[黔科合基础-ZK(2022)一般530];六盘水市科技计划项目(52020-2019-05-05);六盘水师范学院大学生创新创业训练计划项目(S202010977036)。

摘　　要：为明确导致贮藏期红阳猕猴桃果实软腐的病原菌种类,本研究以冷藏期红阳猕猴桃病果为试材,采用组织块分离法分离软腐病病原菌,按照科赫法则确定分离病株的致病性,同时采用形态学结合分子鉴定技术对病原菌种类进行鉴定。结果表明,共有4种软腐病病原菌被鉴定,分别是拟茎点霉菌(Phomopsis sp.)、间座壳菌(Diaporthe sp.)、互隔链格孢菌(Alternaria alternata)和细极链格孢菌(A.tenuissima)。上述4种病原菌是新鉴定的冷藏期红阳猕猴桃果实软腐病的病原菌,其中细极链格孢菌作为猕猴桃果实软腐病病原菌被首次报道。The pathogens were isolated from the decayed Hongyang kiwifruit during cold storage by tissue isolation method followed by Koch′s postulates pathogenicity test.In this study,phenotypic and molecular techniques were applied to characterize the causal agents of Hongyang kiwifruit rot.Results showed a total of four pathogenic fungi were identified,including Phomopsis sp.,Diaporthe sp.,Alternaria alternata and A.tenuissima,which became the new pathogens of Hongyang kiwifruit during storage.Especially,this is the first proof that A.tenuissima as the causal agents can cause the decay of kiwifruit.

关 键 词：红阳猕猴桃 软腐病 病原菌 冷藏期 

分 类 号：S436.634.11[农业科学—农业昆虫与害虫防治]