eIF2B1在肝细胞癌中的表达及意义  

Expression and significance of eIF2B1 in hepatocellular carcinoma

作　　者：齐文月 周大臣[2] 张振华[1] 夏国美[1] 陈伟[2] 邹桂舟[1] Qi Wenyue;Zhou Dachen;Zhang Zhenhua;Xia Guomei;Chen Wei;Zou Guizhou(Dept of Hepatology,the Second Affiliated Hospital of Anhui Medical University,Hefei 230601;Dept of Hepatobiliary Surgery,the Second Affiliated Hospital of Anhui Medical University,Hefei 230601)

机构地区：[1]安徽医科大学第二附属医院肝病科,合肥230601 [2]安徽医科大学第二附属医院肝胆外科,合肥230601

出　　处：《安徽医科大学学报》2023年第5期799-805,共7页Acta Universitatis Medicinalis Anhui

基　　金：安徽省自然科学基金(编号:1608085MH162)。

摘　　要：目的探究真核细胞翻译起始因子2B1(eIF2B1)在肝细胞癌(HCC)中的表达与其临床预后的相关性。方法通过对标本库中743例HCC患者的临床数据进行随访,筛选出91例具有完整随访信息的与乙型肝炎病毒(HBV)相关的HCC患者,选择其术后肿瘤组织,同时留取距肿物边缘>2 cm的癌旁组织,制作为组织芯片,进行Western blot和免疫组化实验,使用Image J分析组织芯片染色的阳性细胞比例及Western blot结果的灰度值,通过R4.0.5软件对纳入患者的临床数据和随访数据进行统计学分析。结果免疫组化结果提示相对于癌旁组织中的表达,eIF2B1在肿瘤组织中的表达效果更强。Western blot结果显示,与HepG2细胞比较,eIF2B1在HepG2.2.15细胞中表达更强,HCC组织中eIF2B1的表达与肿瘤数目相关(P<0.05)。Cox多因素回归分析结果显示,eIF2B1的表达是HBV-DNA阳性患者总体临床预后的独立危险因素(HR=4.28,P=0.018)。结论在HBV相关的HCC组织中,eIF2B1的表达显著增强且与HBV DNA阳性的患者的整体生存显著相关。Objective To investigate the expression of eukaryotic translation initiation factor 2B1(eIF2B1)in HCC and its correlation with clinical prognosis.Methods Through the follow-up of the clinical data of 743 hepatocellular carcinoma patients in the specimen bank,91 HBV-related liver cancer patients with complete follow-up information were screened out,and their postoperative tumor tissues were selected.Meanwhile,the adjacent tumor tissues with a distance of more than 2 cm from the tumor margin were collected to make tissue chips.Western blot and immunohistochemical experiments were performed.Image J was used to analyze the proportion of positive cells stained by tissue chip and the gray value of Western blot results.Clinical data and follow-up data of included patients were statistically analyzed by R4.0.5 software.Results Immunohistochemical results suggested that eIF2B1 was more strongly expressed in HCC than in para-cancerous tissues.Western blot results showed that eIF2B1 was more strongly expressed in HepG2.2.15 cells than in HepG2 cells.The expression of eIF2B1 in HCC tissues was correlated with tumor number(P<0.05).Cox multivariate regression analysis showed that the expression of eIF2B1 was an independent risk factor for the overall clinical prognosis of HBV-DNA positive patients(HR=4.28,P=0.018).Conclusion The expression of eIF2B1 is significantly enhanced in HBV-associated HCC tissues and is significantly associated with overall survival in HBV DNA positive patients.

关 键 词：肝细胞癌 乙型肝炎病毒 eIF2B1 EIF2Α 蛋白质翻译 预后 

分 类 号：R735.7[医药卫生—肿瘤]