海洋贝莱斯芽胞杆菌Bam-6全基因组测序及生物信息分析  

Sequencing and Bioinformatics Analysis of the Whole Genome of Marine Bacillus velezensis Bam-6

作　　者：王金昌[1] WANG Jinchang(Institute of Microbiology Jiangxi Academy of Sciences,Nanchang 330096,China)

机构地区：[1]江西省科学院微生物研究所,南昌330096

出　　处：《中国生物防治学报》2023年第2期438-452,共15页Chinese Journal of Biological Control

基　　金：江西省重点研发项目(20202BBFL63050);江西省科学院包干制试点示范项目(2021YSBG21013)。

摘　　要：贝莱斯芽胞杆菌Bam-6对柑橘溃疡病等植物病原菌具有较强抗菌活性,同时具有杀灭桃蚜的作用,为挖掘Bam-6特殊功能基因及基因簇,深入研究其抑菌杀虫机制,采用第二代BGISEQ与第三代Pac Bio平台相结合的测序技术对Bam-6进行全基因组测序,并对测序数据进行基因组组装、基因预测与功能注释、共线性分析、次级代谢产物合成基因簇预测和比较基因组分析等。Bam-6全基因组全长为3928774 bp,GC含量为46.53%,共编码3861个ORF,含有86个t RNA基因、27个r RNA、0个s RNA、122个串联重复序列和2个卫星RNA。在NR、Swiss-Prot、eggNOG、GO和KEGG数据库分别注释到基因3840、2156、3485、3319和2721个。同时,生物信息学分析表明此菌株基因组中有12个次级代谢产物基因簇,包括3个未知功能的基因簇、8个相似度较高的抗生素合成基因簇(surfactin、macrolactin H、fengycin、bacillaene、difficidin、bacillibactin、bacilysin和mersacidin)和1个相似度仅为7%的抗生素基因簇(butirosinA/B)。与其它贝莱斯芽胞杆菌相比,Bam-6中存在许多相似度低的ORFs,有22个特异基因簇,有2个特有单拷贝基因分别编码类细菌素WGxF蛋白和乳球菌素972。本研究为解析菌株Bam-6的抑菌杀蚜机制从基因组层面上提供了基础数据,为深入了解贝莱斯芽胞杆菌次级代谢产物的合成途径提供提供参考信息,对菌株Bam-6后续相关研究具有重要意义。Bacillus velezensis Bam-6 had strong inhibitory activity against bacterial citrus canker and other plant pathogens and had ability to eliminate peach aphids,in order to explore Bam-6 special functional genes and gene clusters,and further study its antibacterial and insecticidal mechanism,the whole genome sequencing was performed using the BGISEQ II and PacBio III platforms,and the sequencing data were employed for genome assembly,gene prediction and functional annotation,collinearity analysis,secondary metabolite synthesis gene cluster prediction,and comparative genome analysis.The whole genome of Bam-6 is 3,928,774 bp in length with a GC content of 46.53%,potentially producing 3,861 ORFs,and the genome has a total of 86 tRNA,28 rRNA,0 sRNA,122 tandem repeats,and 2 satellite DNA.Up to 3840,2156,3485,3319,or 2721 potential genes were annotated in the NR,Swiss-Prot,eggNOG,Gene Ontology,or Kyoto Encyclopedia of Genes and Genomes databases,respectively.Besides,12 secondary metabolite gene clusters were predicted,including 3 gene clusters with unknown functions,8 antibiotic synthesis gene clusters with high similarity(surfactin,macroactin H,fengycin,bacillaene,difficidin,bacillibactin,bacilysin,and mersacidin),and 1 antibiotic gene cluster with only 7%similarity(butirosin A/B).Compared with other B.velezensis strains,Bam-6 has many ORFs with low similarity,22 specific gene clusters,and 2 unique single-copy genes that encode bacteriocin-like WGxF protein and lactococcin 972,respectively.The present study provides basic data for analyzing the internal factors of the antibacterial and aphid-killing mechanisms of the Bam-6 strain at the genomic level and presents reference information for further understanding the secondary metabolic synthesis pathway of B.velezensis.These results are of great significance for further research on the Bam-6 strain.

关 键 词：贝莱斯芽胞杆菌 基因组 基因注释 次级代谢 

分 类 号：S476[农业科学—农业昆虫与害虫防治]