三种扇贝种间血液生理指标比较及热胁迫对栉孔扇贝血液生理指标的影响  

Comparison of blood physiological parameters between three species of scallops and the effect of heat stress on blood physiological parameters in Chlamys farreri

作　　者：崔畅 杨祖晶 窦峥 陆维[1] 邢强 黄晓婷[1,2] 胡景杰 包振民[1,2,3] CUI Chang;YANG Zujing;DOU Zheng;LU Wei;XING Qiang;HUANG Xiaoting;HU Jingjie;BAO Zhenmin(MOE Key Laboratory of Marine Genetics and Breeding,College of Marine Life Sciences,Ocean University of China,Qingdao 266003,China;Pilot National Laboratory for Marine Science and Technology(Qingdao),Laboratory for Marine Fisheries Science and Food Production Processes,Qingdao 266237,China;Tropical Marine Biological Germplasm Resources Development and Seed Industry Engineering Laboratory,Sanya Oceanographic Institution,Ocean University of China,Sanya 572000,China)

机构地区：[1]中国海洋大学海洋生命学院,海洋生物遗传学与育种教育部重点实验室,山东青岛266003 [2]青岛海洋科学与技术国家实验室,海洋渔业科学与食物产出过程功能实验室,山东青岛266237 [3]中国海洋大学三亚海洋研究院,热带海洋生物种质资源开发与种业工程实验室,海南三亚572000

出　　处：《水产学报》2023年第5期73-81,共9页Journal of Fisheries of China

基　　金：国家重点研发计划(2018YFD0900304);国家现代农业产业技术体系专项;三亚崖州湾科技城管理局专项(SKJC-KJ-2019KY01)。

摘　　要：为了比较3种扇贝血液中的K^(+)、Na^(+)、Ca^(2+)和Cl−浓度,血氧(pO_(2))和血二氧化碳(pCO_(2))气体分压及血液酸碱度(pH)的差异情况,实验利用血气分析仪对栉孔扇贝、虾夷扇贝和海湾扇贝的血液生理指标进行测定。结果显示,扇贝的血液生理指标呈现显著的物种差异,其中栉孔扇贝血液的K^(+)浓度[(15.74±1.47)mmol/L]、Na^(+)浓度[(388.07±11.38)mmol/L]、Cl^(−)浓度[(462.43±6.88)mmol/L]和pO_(2)[(140.13±15.35)mmHg]最高。此外,栉孔扇贝和虾夷扇贝的Ca2+浓度和pCO_(2)含量较高,而海湾扇贝的pH最高。对相同月龄不同大小栉孔扇贝血液指标进行比较,发现栉孔扇贝的血液pH值与其壳高呈正相关(r=0.611),而生理指标与壳高呈负相关。随着温度的升高,栉孔扇贝血液的Ca2+浓度显著增加,而pO_(2)显著下降,K^(+)、Na^(+)和Cl^(−)浓度先升高后恢复到初始水平,而pH和pCO_(2)保持相对稳定。研究结果为贝类血液生理研究提供参考,也为贝类生理状态的评估提供了一种新的思路与方法。Blood physiological parameters reflect the acid-base balance status and oxygen supply of organism by the ion concentration and partial pressure of gas in blood,and play an important role in the animal physiology and pathology.In order to compare three scallop species in terms of blood concentrations of K^(+),Na^(+),Ca2^(+) and Cl^(−),partial blood pressures of O_(2)(pO_(2)) and CO_(2)(pCO_(2)),and blood acidity(pH),a blood gas analyzer was used to measure the blood physiological parameters of Chlamys farreri,Patinopecten yessoensis and Argopecten irradias.The results revealed that there were significant differences in blood physiological parameters between different species of scallops.Specifically,the values of K^(+)[(15.74±1.47)mmol/L],Na^(+)[(388.07±11.38)mmol/L],Cl−concentrations[(462.43±6.88)mmol/L]and pO_(2)[(140.13±15.35)mmHg]were higher in the blood of C.farreri than other two scallops.The values of Ca^(2(+) and pCO_(2) of P.yessoensis and C.farreri were higher than those of A.irradias,and the pH of A.irradias was highest among the three scallops.In addition,the blood pH of C.farreri was significantly positively correlated with shell height(r=0.611,P<0.05),while other physiological parameters were negatively correlated with it.With the increase of temperature,the concentration of Ca2^(+)increased significantly in the blood of C.farreri while pO_(2) decreased significantly.During the temperature change,the concentrations of K^(+),Na^(+) and Cl−in C.farreri increased firstly and then recovered to the initial levels,while the values of pH and pCO_(2) remained relatively stable.The results provide basic information for blood physiology research and a new approach to the assessment of the physiological states of bivalves.

关 键 词：扇贝 栉孔扇贝 血液 离子浓度 气体分压 酸碱平衡 热胁迫 

分 类 号：Q955[生物学—动物学] S968.3[农业科学—水产养殖]