Microbial community structure analyses and cultivable denitrifier isolation of Myriophyllum aquaticum constructed wetland under low C/N ratio  

作　　者：Jialiang Zuo Lina Xu Jianlin Guo Shengjun Xu Shuanglong Ma Cancan Jiang Dongmin Yang Danhua Wang Xuliang Zhuang 

机构地区：[1]Key Laboratory of Environmental Biotechnology,Research Center for Eco-Environmental Sciences,Chinese Academy of Sciences,Beijing 100085,China [2]Sino-Danish Center,University of Chinese Academy of Sciences,Beijing 101408,China [3]Institute of Tibetan Plateau Research,Chinese Academy of Sciences,Beijing 100101,China [4]College of Resources and Environmental Sciences,Henan Agricultural University,Zhengzhou 450002,China [5]Ningxia Zhongke Jingke Testing Technology Company,Yinchuan 750000,China

出　　处：《Journal of Environmental Sciences》2023年第5期30-41,共12页环境科学学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(Nos.42177099 and 91951108);the Key R&D plan of Ningxia Hui Autonomous Region(No.2019BFG02032);the CAS International Partnership Program(No.121311KYSB20200017).

摘　　要：With the rapid expansion of livestock production,the amount of livestock wastewater accumulated rapidly.Lack of biodegradable organic matter makes denitrification of livestock wastewater after anaerobic digestion more difficult.In this study,Myriophyllum aquaticum constructed wetlands(CWs)with efficient nitrogen removal performance were established under different carbon/nitrogen(C/N)ratios.Analysis of community composition reveals the change of M.aquaticum CWs in microbial community structure with C/N ratios.The proportion of Proteobacteria which is one of the dominant phyla among denitrifier communities increased significantly under low C/N ratio conditions.Besides,to obtain cultivable denitrifier that could be added into CWs in situ,33 strains belonging to phylum Proteobacteria were isolated from efficient M.aquaticum CWs,while the best-performing denitrification strain M3-1 was identified as Bacillus velezensis JT3-1(GenBank No.CP032506.1).Redundancy analysis and quadratic models showed that C/N ratio had significant effects on disposal of nitrate(NO_(3)^(−)-N)and the strains isolated could perform well in denitrification when C/N ratio is relatively low.In addition,they have relatively wide ranges of carbon sources,temperature and a high NO_(3)^(−)removal rate of 9.12 mg/(L·hr)at elevated concentrations of 800 mg/L nitrate.Thus,strains isolated from M.aquaticum CWs with low C/N ratio have a practical application value in the treatment of nitrate-containing wastewater.These denitrifying bacteria could be added to CWs to enhance nitrogen removal efficiency of CWs for livestock wastewater with low C/N ratio in the future.

关 键 词：Myriophyllum aquaticum constructed WETLANDS DENITRIFICATION Microbial community structure Bacillus velezensis 

分 类 号：X172[环境科学与工程—环境科学] X713