Triclosan toxicity in a model cyanobacterium (Anabaena flos-aquae): Growth, photosynthesis and transcriptomic response  

作　　者：Jiezhang Mo Linrong Han Runnan Lv Michael W.L.Chiang Rong Fan Jiahua Guo 

机构地区：[1]Shaanxi Key Laboratory of Earth Surface System and Environmental Carrying Capacity,College of Urban and Environmental Sciences,Northwest University,Xi’an 710127,China [2]State Key Laboratory of Marine Pollution and Department of Chemistry,City University of Hong Kong,Kowloon,Hong Kong SAR,China

出　　处：《Journal of Environmental Sciences》2023年第5期82-90,共9页环境科学学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(No.42101077);the Key Research and Development Program of ShaanXi Province(No.2020SF-387);ShaanXi Thousand Talent Program for Young Outstanding Scientists(No.334041900007).

摘　　要：Exposure to triclosan(TCS)has been reported to reduce photosynthetic pigments,suppress photosynthesis,and inhibit growth in both prokaryotic and eukaryotic algae including Anabaena flos-aquae(a model cyanobacterium).In particular,cyanobacteria are more sensitive to TCS toxicity compared to eukaryotic algae possibly due to the structural similarity to bacteria(target organisms);however,whether TCS exerts its toxicity to cyanobacteria by targeting signaling pathways of fatty acid biosynthesis as in bacteria remains virtually unknown,particularly at environmental exposure levels.With the complete genome sequence of A.flos-aquae presented in this study,the transcriptomic alterations and potential toxic mechanisms in A.flos-aquae under TCS stress were revealed.The growth,pigments and photosynthetic activity of A.flos-aquae were markedly suppressed following a 7-day TCS exposure at 0.5μg/L but not 0.1μg/L(both concentrations applied are environmentally relevant).The transcriptomic sequencing analysis showed that signaling pathways,such as biofilm formation–Pseudomonas aeruginosa,two-component system,starch and sucrose metabolism,and photosynthesis were closely related to the TCS-induced growth inhibition in the 0.5μg/L TCS treatment.Photosynthesis systems and potentially two-component system were identified to be sensitive targets of TCS toxicity in A.flos-aquae.The present study provides novel insights on TCS toxicity at the transcriptomic level in A.flos-aquae.

关 键 词：Genome TRANSCRIPTOME Photosynthesis inhibition Two-component system Antimicrobial toxicity 

分 类 号：X171.5[环境科学与工程—环境科学]