Effect of sulfamethazine on the horizontal transfer of plasmid-mediated antibiotic resistance genes and its mechanism of action  

作　　者：Xiaojing Yan Wenwen Liu Shengfang Wen Lanjun Wang Lusheng Zhu Jun Wang Young Mo Kim Jinhua Wang 

机构地区：[1]National Engineering Laboratory for Efficient Utilization of Soil and Fertilizer Resources,Key Laboratory of Agricultural Environment in Universities of Shandong,College of Resources and Environment,Shandong Agricultural University,Tai’an 271018,China [2]Department of Civil and Environmental Engineering,Hanyang University,Seongdong-gu,Seoul 04763,Korea

出　　处：《Journal of Environmental Sciences》2023年第5期399-409,共11页环境科学学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(No.41671320);the Natural Science Foundation of Shandong Province,China(No.ZR2016JL029)and the Special Funds of Taishan Scholar of Shandong Province,China.

摘　　要：As a new type of environmental pollutant,antibiotic resistance genes(ARGs)pose a huge challenge to global health.Horizontal gene transfer(HGT)represents an important route for the spread of ARGs.The widespread use of sulfamethazine(SM2)as a broad-spectrum bacteriostatic agent leads to high residual levels in the environment,thereby increasing the spread of ARGs.Therefore,we chose to study the effect of SM2 on the HGT of ARGs mediated by plasmid RP4 from Escherichia coli(E.coli)HB101 to E.coli NK5449 as well as its mechanism of action.The results showed that compared with the control group,SM2 at concentrations of 10 mg/L and 200 mg/L promoted the HGT of ARGs,but transfer frequency decreased at concentrations of 100 mg/L and 500 mg/L.The transfer frequency at 200 mg/L was 3.04×10^(−5),which was 1.34-fold of the control group.The mechanism of SM2 improving conjugation transfer is via enhancement of the mRNA expression of conjugation genes(trbBP,trfAP)and oxidative stress genes,inhibition of the mRNA expression of vertical transfer genes,up regulation of the outer membrane protein genes(ompC,ompA),promotion of the formation of cell pores,and improvement of the permeability of cell membrane to promote the conjugation transfer of plasmid RP4.The results of this study provide theoretical support for studying the spread of ARGs in the environment.

关 键 词：SULFAMETHAZINE PLASMID Antibiotic resistance gene Conjugation transfer 

分 类 号：X50[环境科学与工程—环境工程]