Toxicity assessment of polyethylene microplastics in combination with a mix of emerging pollutants on Physalaemus cuvieri tadpoles  

作　　者：Amanda Pereira da Costa Araújo Thiarlen Marinho da Luz Mohamed Ahmed Ibrahim Ahmed Mir Mohammad Ali Md.Mostafizur Rahman Bojan Nataraj Daniela de Melo e Silva DamiàBarceló Guilherme Malafaia 

机构地区：[1]Post-Graduation Program in Environmental Sciences,Federal University of Goiás,Goiania,GO 74690-970,Brazil [2]Laboratory of Toxicology Applied to the Environment,Goiano Federal Institute,Urutaí,GO 75790-000,Brazil [3]Plant Protection Department,Faculty of Agriculture,Assiut University,Assiut 71526,Egypt [4]Department of Aquaculture,Faculty of Fisheries,Aquaculture&Marine Science,Sher-e-Bangla Agricultural University,Dhaka 1207,Bangladesh [5]Department of Environmental Sciences,Jahangirnagar University,Savar 1342,Bangladesh [6]Unit of Toxicology,Department of Zoology,School of Life Sciences,Bharathiar University,Coimbatore 641046,India [7]Laboratory of Environmental Mutagenesis,Federal University of Goiás,Goiania,GO 74690-970,Brazil [8]Catalan Institute for Water Research(ICRA-CERCA),H_(2)O Building,Scientific and Technological Park of the University of Girona,Emili Grahit 101,Girona 17003,Spain [9]Water and Soil Quality Research Group,Department of Environmental Chemistry,Institute of Environmental Assessment and Water Research(IDAEA-CSIC),JordiGirona 1826,Barcelona 08034,Spain [10]Post-Graduation Program in Conservation of Cerrado Natural Resources,Goiano Federal Institute,Urutaí,GO 75790-000,Brazil [11]Post-Graduation Program in Ecology,Conservation,and Biodiversity,Federal University of Uberlandia,Uberlandia,MG 38400-902,Brazil [12]Post-Graduation Programa in Biotechnology and Biodiversity,Federal University of Goiás,Goiania,GO 74605-050,Brazil

出　　处：《Journal of Environmental Sciences》2023年第5期465-482,共18页环境科学学报（英文版）

基　　金：supporting the financing of this study(Process#307743/2018–7);the granting of research productivity grants to Dr.Malafaia G.(Proc.#307743/2018–7)and Dr.Melo e Silva D.(Process#307652/2018-1);for the scientific initiation scholarships to students Silva A.M.and Luz T.M.In addition,the authors thank the Federal Institute of Goiano(GO,Brazil);granting important financial assistance for the publication of this study(Proc.#23219.000139.2022-17).

摘　　要：Studies in recent years have shown that aquatic pollution by microplastics(MPs)can be considered to pose additional stress to amphibian populations.However,our knowledge of how MPs affect amphibians is very rudimentary,and even more limited is our understanding of their effects in combination with other emerging pollutants.Thus,we aimed to evaluate the possible toxicity of polyethylene MPs(PE-MPs)(alone or in combination with a mix of pollutants)on the health of Physalaemus cuvieri tadpoles.After 30 days of exposure,multiple biomarkers were measured,including morphological,biometric,and developmental indices,behavioral parameters,mutagenicity,cytotoxicity,antioxidant and cholinesterase responses,as well as the uptake and accumulation of PE-MPs in animals.Based on the results,there was no significant change in any of the parameters measured in tadpoles exposed to treatments,but induced stress was observed in tadpoles exposed to PE-MPs combined with themixture of pollutants,reflecting significant changes in physiological and biochemical responses.Through principal component analysis(PCA)and integrated biomarker response(IBR)assessment,effects induced by pollutants in each test group were distinguished,confirming that the exposure of P.cuvieri tadpoles to the PE-MPs in combination with a mix of emerging pollutants induces an enhanced stress response,although the uptake and accumulation of PE-MPs in these animalswas reduced.Thus,our study provides newinsight into the danger to amphibians of MPs coexisting with other pollutants in aquatic environments.

关 键 词：AMPHIBIANS Environmental toxicology MICROPOLLUTANTS Emerging pollutants Aquatic pollution Biomarkers 

分 类 号：X171.5[环境科学与工程—环境科学]