Seasonal variations of microbial community and antibiotic resistome in a suburb drinking water distribution system in a northern Chinese city  

作　　者：Yanchu Ke Wenjun Sun Zibo Jing Zhinan Zhao Shuguang Xie 

机构地区：[1]State Key Joint Laboratory of Environmental Simulation and Pollution Control,College of Environmental Sciences and Engineering,Peking University,Beijing 100871,China [2]School of Environment,Tsinghua University,Beijing 100084,China [3]Research Institute for Environmental Innovation(Suzhou)Tsinghua,Suzhou 215163,China

出　　处：《Journal of Environmental Sciences》2023年第5期714-725,共12页环境科学学报（英文版）

基　　金：supported by the National Key R&D Program of China(No.2019YFC0408700).

摘　　要：Antibiotic resistance genes(ARGs)are an emerging issue for drinkingwater safety.However,the seasonal variation of ARGs in drinking water distribution systems(DWDS)is still unclear.This work revealed the tempo-spatial changes of microbial community,ARGs,mobile genetic elements(MGEs)co-occurring with ARGs,ARG hosts in DWDS bulk water by means of metagenome assembly.The microbial community and antibiotic resistome varied with sampling season and site.Temperature,ammonia,chlorite and total plate count(TPC)drove the variations of microbial community structure.Moreover,environmental parameters(total organic carbon(TOC),chlorite,TPC and hardness)shifted antibiotic resistome.ARGs and MGEs co-occurring with ARGs showed higher relative abundance in summer and autumn,which might be attributed to detached pipe biofilm.In particular,ARG-bacitracin and plasmid were the predominant ARG and MGE,respectively.ARG hosts changed with season and site and were more diverse in summer and autumn.In winter and spring,Limnohabitans and Mycobacterium were the major ARG hosts as well as the dominant genera in microbial community.In addition,in summer and autumn,high relative abundance of Achromobacter and Stenotrophomonas were the hosts harboring many kinds of ARGs and MGEs at site in a residential zone(0.4 km from the water treatment plant).Compared with MGEs,microbial community had a greater contribution to the variation of antibiotic resistome.This work gives new insights into the dynamics of ARGs in full-scale DWDS and the underlying factors.

关 键 词：Drinking water distribution system Seasonal change Microbial community Antibiotic resistance genes Mobile genetic elements 

分 类 号：TU991.25[建筑科学—市政工程] X172[环境科学与工程—环境科学]