Reproductive toxicity of enrofloxacin in Caenorhabditis elegans involves oxidative stress-induced cell apoptosis  

作　　者：Jiahao Huang Lizi Liao Guowei Wang Zhongkun Du Zhengxing Wu 

机构地区：[1]Key Laboratory of Molecular Biophysics,Ministry of Education,College of Life Science and Technology,Huazhong University of Science and Technology,Wuhan 430074,China [2]School of Environmental Ecology and Biological Engineering,Wuhan Institute of Technology,Wuhan 430205,China [3]Key Laboratory of Green Chemical Engineering Process of Ministry of Education,Wuhan 430205,China [4]College of Resources and Environment,Shandong Agricultural University,Taian 271018,China

出　　处：《Journal of Environmental Sciences》2023年第5期726-737,共12页环境科学学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(No.21806047);the National Postdoctoral Program for Innovative Talents of China(No.BX201700310);the Postdoctoral Science Foundation of China(No.2018M632869);the 16th University President Fund of Wuhan University of Technology(No.XZJJ2021105).

摘　　要：Fluoroquinolone antibiotics(FQs)that persist and bioaccumulate in the environment have aroused people’s great concern.Here,we studied the adverse effects of FQs in soil animals of Caenorhabditis elegans via food-chronically exposure.The result shows C.elegans exposed to FQs exhibited reproductive toxicity with small-brood size and low-egg hatchability.To study the underlying mechanism,we conduct a deep investigation of enrofloxacin(ENR),one of the most frequently detected FQs,on nematodes which is one of commonly used animal indicator of soil sustainability.The concentration-effect curves simulated by the Hill model showed that the half effect concentrations(EC50)of ENR were(494.3±272.9)μmol/kg and(107.4±30.9)μmol/kg for the brood size and the hatchability,respectively.Differential gene expression between the control and the ENR-exposure group enriched with the oxidative stress and cell apoptosis pathways.The results together with the enzyme activity in oxidative stress and the cell corpses suggested that ENR-induced reproductive toxicity was related to germ cell apoptosis under oxidative stress.The risk quotients of some soil and livestock samples were calculated based on the threshold value of EC10 for the egg hatchability(2.65μmol/kg).The results indicated that there was possible reproductive toxicity on the nematodes in certain agricultural soils for the FQs.This study suggested that chronic exposure to FQs at certain levels in environment would induce reproductive toxicity to the nematodes and might reduce the soil sustainability,alarming the environment risks of antibiotics abuse.

关 键 词：Environmental toxicity Nematodes Chronic exposure Oxidative stress response Germ cell apoptosis 

分 类 号：X171.5[环境科学与工程—环境科学]