Chlorine substitution-dependent toxicities of polychlorinated biphenyls to the earthworm Eisenia fetida in soil  

作　　者：Jianying Zhang Mengyang He Yaoxuan Liu Lei Zhang Haojie Jiang Daohui Lin 

机构地区：[1]College of Environmental and Resource Sciences,Zhejiang University,Hangzhou 310058,China [2]National Demonstration Center for Experimental Environment and Resources Education,Zhejiang University,Hangzhou 310058,China [3]Institute of Environmental Health,Zhejiang University,Hangzhou 310058,China [4]Zhejiang Provincial Key Laboratory of Organic Pollution Process and Control,Hangzhou 310058,China [5]China Energy Science and Technology Research Institute Co.Ltd.,Nanjing 210023,China

出　　处：《Journal of Environmental Sciences》2023年第6期171-180,共10页环境科学学报（英文版）

基　　金：This work was supported by the National Key Research and Development Program of China(No.2017YFA0207003);the National Natural Science Foundation of China(No.21777139).

摘　　要：Polychlorinated biphenyls(PCBs)with different chlorine substitution patterns often coexist in e-waste-processing sites.However,the single and combined toxicity of PCBs to soil organisms and the influence of chlorine substitution patterns remain largely unknown.Herein,we evaluated the distinct in vivo toxicity of PCB28(a trichlorinated PCB),PCB52(a tetrachlorinated PCB),PCB101(a pentachlorinated PCB),and their mixture to earthworm Eisenia fetida in soil,and looked into the underlining mechanisms in an in vitro test using coelomocytes.After a 28-days exposure,all PCBs(up to 10 mg/kg)were not fatal to earthworms,but could induce intestinal histopathological changes and microbial community alterations in the drilosphere system,along with a significant weight loss.Notably,pentachlorinated PCBs with a low bioaccumulation ability showed greater inhibitory effects on the growth of earthworm than lowly chlorinated PCBs,suggesting that bioaccumulation was not the main determinant of chlorine substitution-dependent toxicity.Furthermore,in vitro assays showed that the highly chlorinated PCBs induced a high-percentage apoptosis of eleocytes in the coelomocytes and significantly activated antioxidant enzymes,indicating that the distinct cellular vulnerability to lowly/highly chlorinated PCBs was the main contributor to the PCBs toxicity.These findings emphasize the specific advantage of using earthworms in the control of lowly chlorinated PCBs in soil due to their high tolerance and accumulation ability.

关 键 词：Soil contamination ORGANOCHLORINE INVERTEBRATE Subchronic toxicity COELOMOCYTE 

分 类 号：X171.5[环境科学与工程—环境科学]