Coastal mudflats as reservoirs of extracellular antibiotic resistance genes: Studies in Eastern China  

作　　者：Qingbin Yuan Xiaolin Wang Hui Fang Yuan Cheng Ruonan Sun Yi Luo 

机构地区：[1]College of Environmental Science and Engineering,Nanjing Tech University,Nanjing 211816,China [2]State Key Laboratory of Pollution Control and Resource Reuse,School of the Environment,Nanjing University,Nanjing 210023,China [3]School of Medicine,Nankai University,Tianjin 300071,China [4]Department of Civil and Environmental Engineering,Rice University,Houston 77005,USA

出　　处：《Journal of Environmental Sciences》2023年第7期58-68,共11页环境科学学报（英文版）

基　　金：This study was supported by the National Natural Science Foundation of China(No.42177348);the Natural Science Foundation of Jiangsu Province(No.BK20201367);China Postdoctoral Science Foundation(No.2021M701663).

摘　　要：Despite coastal mudflats serving as essential ecological zones interconnecting terrestrial/freshwater and marine systems,little is known about the profiles of antibiotic resistance genes(ARGs)in this area.In this study,characteristics of typical ARGs,involving both intracellular(iARGs)and extracellular ARGs(eARGs)at different physical states,were explored in over 1000 km of coastal mudflats in Eastern China.Results indicated the presence of iARGs and eARGs at states of both freely present or attached by particles.The abundance of eARGs was significantly higher than that of iARGs(87.3%vs 12.7%),and their dominance was more significant than those in other habitats(52.7%-76.3%).ARG abundance,especially for eARGs,showed an increasing trend(p<0.05)from southern(Nantong)to northern(Lianyungang)coastalmudflats.Higher salinity facilitated the transformation from iARGs to eARGs,and smaller soil particle size was conducive to the persistence of eARGs in northern coastal mudflats.This study addresses the neglected function of coastal mudflats as eARGs reservoirs.

关 键 词：Antibiotic resistance genes(ARGs) Extracellular antibiotic resistance genes(eARGs) Coastal mudflats Amplicon sequencing Geochemical variable Mobile genetic elements 

分 类 号：X172[环境科学与工程—环境科学]