水溶性蜂胶对溃疡性结肠炎大鼠肠系膜动脉内皮的保护作用  

Protective effect of water-soluble propolis on mesenteric artery endothelium in rats with ulcerative colitis

作　　者：李慧[1] 张浩 陈朋 魏翔 方莹 王静[1] 陈雪祎 孙丽[1] 王海华[1] LI Hui;ZHANG Hao;CHEN Peng;WEI Xiang;FANG Ying;WANG Jing;CHEN Xueyi;SUN Li;WANG Haihua(Department of Physiology,Wannan Medical College,Wuhu 241002,China)

机构地区：[1]皖南医学院生理学教研室,安徽芜湖241002 [2]皖南医学院临床医学院,安徽芜湖241002 [3]皖南医学院药学院,安徽芜湖241002

出　　处：《皖南医学院学报》2023年第2期112-115,共4页Journal of Wannan Medical College

基　　金：安徽高校自然科学研究重点项目(KJ2016A729,KJ2020A0606);国家级大学生创新创业训练计划项目(202110368034)。

摘　　要：目的:探究水溶性蜂胶(WSP)对溃疡性结肠炎(UC)大鼠肠系膜动脉血管内皮功能的影响。方法:SD雄性大鼠随机分成4组(n=6),即正常对照组(NC组)、溃疡性结肠炎组(DSS组)、蜂胶低剂量组(L-WSP组)、蜂胶高剂量组(H-WSP组)。测量并记录各组大鼠结肠长度,HE染色观察结肠组织形态学变化。张力测定仪检测离体肠系膜动脉舒张功能。肠系膜血管HE染色并分析血管壁厚度/管腔半径(WT/LR)。检测肠系膜动脉中内皮型一氧化氮合酶(eNOS)活性和一氧化氮(NO)含量。结果:与DSS组相比,L-WSP组和H-WSP组大鼠结肠长度增长(P<0.05),结肠组织的结构和形态损伤减轻,肠系膜动脉血管壁变薄,WT/LR下降(P<0.01),肠系膜动脉对乙酰胆碱(ACh)介导的舒张反应减弱(P<0.01),肠系膜动脉eNOS活性和NO含量增加(P<0.01);经左旋硝基精氨酸甲酯(L-NAME)孵育30 min后,肠系膜动脉对ACh介导的舒张反应减弱(P<0.05)。结论:WSP能保护UC大鼠肠系膜动脉内皮损伤并改善动脉舒张功能,其机制可能与调节eNOS/NO信号通路有关。Objective:To investigate the effect of water soluble propolis(WSP)on vascular endothelial function of mesenteric artery in rats with ulcerative colitis(UC).Methods:Male SD rats were randomly divided into normal control group(NC group),ulcerative colitis group(DSS group),low WSP dose group(L-WSP group)and high WSP dose group(H-WSP group)(n=6 for each group).The colon length was measured and maintained in rats in each group,and histomorphological changes of the colon were observed by HE staining.Tensiometer was used to determine diastolic function of the isolated mesenteric artery.Mesenteric vessels were stained by HE for observing the vessel wall thickness/lumen radius(WT/LR).The activity of endothelial nitric oxide synthase(eNOS)and the content of nitric oxide(NO)in mesenteric artery were detected.Results:Compared with DSS group,rats in L-WSP and H-WSP group had increased length of the colon(P<0.05),decreased damage to the colon tissue both structurally and morphologically,thinned vascular wall of mesenteric artery,reduced ratio of WT/LR(P<0.01),improved relaxation response of mesenteric artery mediated by ACh(P<0.01),and decreased eNOS activity and NO content of mesenteric artery(P<0.01).After incubation with N-nitro-L-arginine-methyl-ester(L-NAME)for 30 min,the relaxation of mesenteric artery mediated by ACh was weakened(P<0.05).Conclusion:WSP can protect the endothelium of the mesenteric artery from injury and improve arterial diastolic function in UC rats.The mechanism may be related to regulation of eNOS/NO signaling pathway.

关 键 词：水溶性蜂胶 溃疡性结肠炎 肠系膜动脉 内皮型一氧化氮合酶 一氧化氮 

分 类 号：R574.62[医药卫生—消化系统] R-332[医药卫生—内科学] R285.5[医药卫生—临床医学]