猪肉中硝基呋喃代谢物液质检测法的优化及衍生条件探究  

Optimization of Nitrofuran Detection and Derivative Conditions in Pork

作　　者：黄立 HUANG Li(Kunming Institute for Food and Drug Control,Kunming 650032,China)

机构地区：[1]昆明市食品药品检验所,云南昆明650032

出　　处：《食品安全导刊》2023年第13期50-54,共5页China Food Safety Magazine

摘　　要：目的:对国标法(GB/T 21311—2007)猪肉中硝基呋喃代谢物残留量检测方法进行提取净化步骤优化并对其衍生剂体积和衍生时间进行探究。方法:样品经衍生提取后,对比通过HyperSep Retain PEP小柱进行净化和国标法净化后图谱的基线噪音及峰面积响应情况;采用标准溶液衍生后峰面积的响应值对衍生剂用量、衍生时间进行探究。结果:衍生剂体积的量仅对呋喃妥因代谢物和呋喃西林代谢物影响大,随着衍生剂体积的增加,衍生效率增强;衍生时间4 h及以上即可满足检测要求;采用HyperSep Retain PEP小柱净化基线相对较低平、噪音较低,方法检出限为0.5μg·kg^(-1),加标回收率为89.8%~109.6%,RSD为0.7%~2.0%。结论:该方法处理过程简洁、省时,测定结果准确,数据可靠,可实现对猪肉中硝基呋喃代谢物的快速检测。Objective:To optimize the extraction and purification procedure of the national standard method(GB/T 21311-2007)for detecting residual nitrofuran metabolites in pork,and to explore the volume and derivation time of its derivatives.Method:After the samples were derived and extracted,the baseline noise and peak area responses of the atlas after purification by HyperSep Retain PEP column and national standard method were compared.The response value of peak area after derivative of standard solution was used to explore the amount of derivative and derivative time.Result:The derivatives volume only had a significant effect on furantoin metabolites and furacillin metabolites,and the derivatives efficiency increased with the increase of derivatives volume.The derivative time of 4 h and above can meet the detection requirements;HyperSep Retain PEP column was adopted with a relatively low purification baseline and low noise.The detection limit of the method was 0.5μg·kg^(-1),the standard recoveries were 89.8%~109.6%,and RSDS were 0.7%~2.0%.Conclusion:The method is simple,time-saving,accurate and reliable,and can be used for the rapid detection of nitrofuran metabolites in pork.

关 键 词：硝基呋喃代谢物 液质联用仪 衍生剂 净化 

分 类 号：TS251.51[轻工技术与工程—农产品加工及贮藏工程] O657.63[轻工技术与工程—食品科学与工程]