美蓝染色技术在胃镜活检切片组织幽门螺杆菌检查中的应用  

Application of Methylene Blue Staining Technique in the Examination of Helicobacter Pylori in Endoscopic Biopsy Tissues

作　　者：刘小飞[1] 刘艳兰 LIU Xiao-fei;LIU Yan-lan(Department of Pathology,Taihe County People's Hospital,Taihe 343700,Jiangxi,China)

机构地区：[1]泰和县人民医院病理科,江西泰和343700 [2]泰和县妇幼保健院妇产科,江西泰和343700

出　　处：《医学信息》2023年第11期142-144,148,共4页Journal of Medical Information

基　　金：吉安市科技项目(编号:吉市科计字〔2022〕6号序号241)。

摘　　要：目的观察美蓝染色技术在胃镜活检切片组织中检查幽门螺杆菌(Hp)的临床应用价值。方法选取2020年7月-12月在我院进行胃镜活检的50例患者为研究对象,均在胃镜下钳取胃黏膜组织分别进行快速尿酶法(RUT)、美蓝染色技术检测,比较两组检测方法Hp检出率、敏感度、特异度以及染色效果。结果RUT检测Hp阳性率为84.00%,低于美蓝染色检测96.00%,差异有统计学意义(P<0.05);美蓝染色检测Hp敏感度、特异度均高于RUT检测,差异有统计学意义(P<0.05);美蓝染色方法着色强度、细胞阳性率评分均高于RUT检测,差异有统计学意义(P<0.05),但着色背景评分与RUT检测比较,差异无统计学意义(P>0.05);2、5、10 min美蓝染色检测Hp阳性率均高于RUT检测(P<0.05),但20 min时,两者比较差异无统计学意义(P>0.05)。结论美蓝染色技术在胃镜活检切片组织Hp检测中具有重要的作用,可提高阳性检出率,且检出敏感度、特异度均高于RUT检测。同时染色效果显著,利于镜下观察,且受显色反应时间影响相对较小,可一定程度提高Hp检出准确率,为临床早期治疗提供可靠的参考依据。Objective To observe the clinical application value of methylene blue staining technique in the examination of Helicobacter pylori(Hp)in endoscopic biopsy tissue.Methods A total of 50 patients who underwent gastroscopy biopsy in our hospital from July to December 2020 were selected as the research objects.Gastric mucosal tissues were taken under gastroscopy for rapid urease test(RUT)and methylene blue staining.The detection rate,sensitivity,specificity and staining effect of Hp were compared between the two groups.Results The positive rate of Hp detected by RUT was 84.00%,which was lower than 96.00% of methylene blue staining,and the difference was statistically significant(P<0.05).The sensitivity and specificity of Hp detection by methylene blue staining were higher than those of RUT detection,and the differences were statistically significant(P<0.05).The staining intensity and cell positive rate scores of methylene blue staining method were higher than those of RUT detection,and the differences were statistically significant(P<0.05),but the staining background score was compared with that of RUT detection with no significant difference(P>0.05).The positive rate of Hp detected by methylene blue staining at 2,5 and 10 min was higher than that detected by RUT(P<0.05),but there was no significant difference between the two groups at 20 min(P>0.05).Conclusion The methylene blue staining technique plays an important role in the Hp detection of gastroscopy biopsy tissue,which can improve the positive detection rate,and the detection sensitivity and specificity are higher than those of RUT detection.At the same time,the staining effect is significant,which is conducive to microscopic observation,and is relatively less affected by the color reaction time.Meanwhile,it can improve the accuracy of Hp detection to a certain extent and provide a reliable reference for early clinical treatment.

关 键 词：美蓝染色技术 胃镜活检 幽门螺杆菌 快速尿酶法 

分 类 号：R735.2[医药卫生—肿瘤]