基于SRAP标记的不同产区黄精的遗传多样性  

Genetic diversity of Polygonatum spp.from different production areas based on SRAP markers

作　　者：李亚萍[1,2] 戴惠明 姜武 陈家栋[2] 段晓婧 陶正明 LI Yaping;DAI Huiming;JIANG Wu;CHEN Jiadong;DUAN Xiaojing;TAO Zhengming(Provincial Key Laboratory of Conservation and Innovative Utilization of Traditional Chinese Medicine Resources,College of Food and Health,Zhejiang A&F University,Hangzhou 311300,Zhejiang,China;Zhejiang Institute of Subtropical Crop,Wenzhou 325005,Zhejiang,China;Natural Resources and Planning Bureau of Yongjia County,Yongjia 325199,Zhejiang,China)

机构地区：[1]浙江农林大学食品与健康学院浙江省特色中药资源保护与创新利用重点实验室,浙江杭州311300 [2]浙江省亚热带作物研究所,浙江温州325005 [3]永嘉县自然资源和规划局,浙江永嘉325199

出　　处：《浙江农林大学学报》2023年第3期658-664,共7页Journal of Zhejiang A&F University

基　　金：浙江省中药资源保护与创新利用重点实验室项目(2021E10013);温州市农业新品种选育协作组项目(2019ZX003-1)。

摘　　要：【目的】研究不同产区黄精Polygonatum spp.的遗传多样性,为其资源保护及品种选育提供更多理论依据。【方法】使用相关序列扩增多态性(SRAP)分子标记技术,分析来自华东(浙江、福建、安徽、江西)、西北(河北、陕西)、华中(湖南)、西南(四川、贵州、云南)等4个居群的47份黄精种质资源的遗传多样性。【结果】对88对SRAP引物进行筛选,有7对可用于黄精种质资源的SRAP-PCR分析,共得到159条扩增条带,其中多态性条带140条。物种水平上,多态性比率为88.05%,有效等位基因数(Ne)为1.600 6,Nei’s基因多样性指数(H)为0.204 1,Shannon信息指数(I)为0.308 0;居群水平上,多态性比率为42.14%~86.16%,H和I分别为0.188 1~0.259 1和0.238 2~0.399 4;4个居群的基因分化系数(G_(st))为0.194 1,表明有80.59%的遗传变异在种群内进行,基因流(N_(m))为2.075 4,表明居群间存在一定的基因流动;从非加权组平均法(UPGMA)聚类结果可知:当相似系数为0.66时,47份样品分成4组,Ⅰ、Ⅲ、Ⅳ类的均为华东地区浙江种质资源,当相似系数为0.68时,Ⅱ类分成2支。西南与西北聚为一类,浙江庆元黄精聚类结果复杂,表明地理位置差异与亲缘关系远近没有特定关系。整体上华东居群遗传多样性丰富,而庆元黄精种质遗传多样性最丰富。【结论】黄精遗传多样性水平较高,居群间存在一定的基因交流,可为黄精新品种选育工作提供参考。图3表4参22。[Objective]The objective is to study the genetic diversity of Polygonatum spp.from different production areas and provide further theoretical support for its resource protection and variety breeding.[Method]The genetic diversity of 47 Polygonatum spp.germplasm resources from 4 populations,including East(Zhejiang,Fujian,Anhui,Jiangxi),Northwest(Hebei,Shaanxi),Central(Hunan),Southwest(Sichuan,Guizhou and Yunnan)China,was analyzed using related sequence amplification polymorphism(SRAP)molecular markers technology.[Result]A total of 88 pairs of SRAP primers were screened,and 7 pairs could be used for SRAP-PCR analysis of Polygonatum spp..A total of 159 amplified bands were obtained,including 140 polymorphic bands.At the species level,the polymorphism ratio(PPB)was 88.05%,the effective allele number(Ne)was 1.6006,the Nei’s genetic diversity(H)was 0.2041,and Shannon’s information index(I)was 0.3080.At the population level,PPB was 42.14%−86.16%,H and I were 0.1881−0.2591 and 0.2382−0.3994,respectively.The genetic differentiation coefficient(Gst)of the 4 populations was 0.1941,indicating that 80.59%of the genetic variation was carried out within the population,and the gene flow(N_(m))was 2.0754,indicating that there was a certain gene flow between populations.According to the clustering results of the unweighted group average method(UPGMA),when the similarity coefficient was 0.66,47 samples were divided into 4 groups.ClassⅠ,ⅢandⅣwere all Zhejiang germplasm resources in East China.When the similarity coefficient was 0.68,ClassⅡwas divided into 2 branches.Those in Southwest and Northwest were clustered into one class,and the clustering results of Polygonatum spp.in Qingyuan County of Zhejiang Province were complex,indicating that there was no specific relationship between the geographical difference and the distance of kinship.On the whole,the genetic diversity of populations in East China was rich,while Qingyuan had the richest.[Conclusion]The level of genetic diversity is high and there is certain gene

关 键 词：黄精 相关序列扩增多态性(SRAP)分子标记 遗传多样性 产区 

分 类 号：S722[农业科学—林木遗传育种]