白斑综合征病毒(WSSV)灭活制剂对克氏原螯虾抗WSSV的研究  

Anti-WSSV effect of inactivated preparation of white spot syndrome virus(WSSV)on Procambarus clarkii

作　　者：裘杰珂 赖勇勇 许英蕾 朱斐 QIU Jieke;LAI Yongyong;XU Yinglei;ZHU Fei(Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province,College of Animal Science and Technology/College of Veterinary Medicine,Zhejiang A&F University,Hangzhou 311300,Zhejiang,China)

机构地区：[1]浙江农林大学动物科技学院/动物医学院浙江省畜禽绿色生态健康养殖应用技术研究重点实验室,浙江杭州311300

出　　处：《浙江农林大学学报》2023年第3期680-684,共5页Journal of Zhejiang A&F University

基　　金：浙江省自然科学基金面上资助项目(LY20C190001)。

摘　　要：【目的】研究经双乙烯亚胺(BEI)灭活的白斑综合征病毒(WSSV)灭活制剂保护克氏原螯虾Procambarus clarkii抗WSSV感染的效果,以期为白斑综合征的防治提供有效的免疫方法。【方法】应用BEI对WSSV和WSSV超声破碎液进行灭活,通过口服和注射分别对克氏原螯虾进行免疫,再对其进行抗WSSV感染的效果研究。【结果】WSSV在BEI处理24 h可以被完全灭活,通过口服途径用灭活制剂免疫克氏原螯虾7 d后攻毒,克氏原螯虾死亡率显著下降,且口服免疫的效果要好于注射免疫。【结论】经BEI灭活24 h的WSSV灭活制剂对克氏原螯虾是安全的,口服免疫后可以显著降低克氏原螯虾感染白斑综合征病毒的死亡率。图2表3参19。[Objective]This study is to explore the protective effect of white spot syndrome virus(WSSV)preparation inactivated by binary ethyleneimine(BEI)on Procambarus clarkii against WSSV infection,so as to provide an effective immune method for the prevention of WSSV.[Method]Starting with the preparation of WSSV inactivation preparations,BEI was applied to inactivate WSSV,immunize P.clarkii by oral and injection methods,and then conduct anti-WSSV infection test.[Result]WSSV could be completely inactivated under BEI treatment for 24 hours.After 7 days of oral immunization with inactivated WSSV,the mortality rate of P.clarkii was significantly decreased.The effect of oral immunization was better than that of injection immunization.[Conclusion]The inactivated WSSV preparation inactivated by BEI for 24 hours is safe for P.clarkii and can significantly reduce its mortality infected with WSSV.[Ch,2 fig.3 tab.19 ref.]

关 键 词：白斑综合征病毒 双乙烯亚胺(BEI) 灭活 克氏原螯虾 

分 类 号：S945.4[农业科学—水产养殖]