SlZF3 regulates tomato plant height by directly repressing SlGA20ox4 in the gibberellic acid biosynthesis pathway  

作　　者：Jinying Luo Yunfei Tang Zhuannan Chu Yuxin Peng Jiawei Chen Huiyang Yu Chunmei Shi Jahanzeb Jafar Rong Chen Yaping Tang Yongen Lu Zhibiao Ye Ying Li Bo Ouyang 

机构地区：[1]National Key Laboratory for Germplasm Innovation&Utilization of Horticultural Crops,College of Horticulture and Forestry Sciences,Huazhong Agricultural University,Wuhan 430070,China [2]College of Horticulture,Henan Agricultural University,Zhengzhou 450002,China

出　　处：《Horticulture Research》2023年第4期71-82,共12页园艺研究（英文）

基　　金：supported by the National Natural Science Foundation of China(31972416,U1906205,U21A20230);the National Key Research and Development Program of China(2022YFE0100900,2018YFD1000800);the earmarked fund for CARS(CARS-23-A13);the Foundation for Young Talents of Henan Agricultural University(30500728);the Key Scientific Research Project of the Higher Education Institutions of Henan Province(23A210008).

摘　　要：Plant height is an important target trait for crop genetic improvement.Our previous work has identified a salt-tolerant C2H2 zinc finger,SlZF3,and its overexpression lines also showed a semi-dwarf phenotype,but themolecular mechanism remains to be elucidated.Here,we characterized the dwarf phenotype in detail.The dwarfism is caused by a decrease in stem internode cell elongation and deficiency of bioactive gibberellic acids(GAs),and can be rescued by exogenous GA3 treatment.Gene expression assays detected reduced expression of genes in the GA biosynthesis pathway of the overexpression lines,including SlGA20ox4.Several protein-DNA interaction methods confirmed that SlZF3 can directly bind to the SlGA20ox4 promoter and inhibit its expression,and the interaction can also occur for SlKS and SlKO.Overexpression of SlGA20ox4 in the SlZF3-overexpressing line can recover the dwarf phenotype.Therefore,SlZF3 regulates plant height by directly repressing genes in the tomato GA biosynthesis pathway.

关 键 词：DWARF HEIGHT EXPRESSING 

分 类 号：S641.2[农业科学—蔬菜学]