Molecular identification and in vitro interaction of molds associated with dry rot of potato(Solanum tuberosum L.)collected in La Trinidad,Benguet,Philippines  

作　　者：Carreon GAD Gandalera EE Waing KGD 

机构地区：[1]Department of Biological Sciences,College of Science,Central Luzon State University,Science City of Muñoz,Nueva Ecija,Philippines 3120

出　　处：《Studies in Fungi》2021年第1期315-326,共12页真菌研究（英文）

摘　　要：Solanum tuberosum L.from the Solanaceae family is one of the most important agricultural crops grown in the Philippines next to rice.Unfortunately,harvested potatoes were plagued with various fungal diseases resulting to occurrence of dry rot affecting the production and harvest of potatoes.Dry rot in potatoes is caused by several fungal species in the genus Fusarium.Hence,this study aimed to isolate and identify fungal species associated with dry rot disease of potato.Potatoes showing diseased symptoms of dry rot were randomly selected and collected from various traders in La Trinidad,Benguet and were subjected for fungal isolation through serial dilution and plating.Identification was done through observation of cultural and morphological characteristics as well as DNA barcoding using ITS region.A total of six fungal species were subjected into BLAST which revealed the following species:Aspergillus flavus(100.00%),A.fumigatus(100.00%),A.niger(99.82%),Fusarium oxysporum(95.06%),F.solani(100.00%)and Mucor velutinosus(96.45%).To establish possible fungal species as potential biocontrol agent that would lessen the use of harmful chemicals,the identified fungal organisms were interacted with one another through in vitro interaction.Results showed that fungi exhibited antagonistic interaction against each other.Hyphal denaturation,hyphal penetration,hyphal coiling and cell lysis,were observed through microscopic observation.Meanwhile,M.velutinosus showed the ability to penetrate the host hyphae of the F.solani and F.oxysporum.Therefore,Mucor velutinosus could be a potential biological control agent against Fusarium species,which can reduce the use of harmful fungicides in controlling fungal diseases in potato that causes dry rot.

关 键 词：ANTAGONISM fungal diseases hyphal interaction Mucor velutinosus 

分 类 号：S53[农业科学—作物学]