In vitro antioxidant and wound healing activity of Sargassum polycystum hydroethanolic extract in fibroblasts and keratinocytes  

作　　者：Wanwipha Woonnoi Furoida Moolsap Supita Tanasawet Nattakanwadee Khumpirapang Chakkapat Aenglong Wanida Sukketsiri 

机构地区：[1]Division of Health and Applied Sciences,Faculty of Science,Prince of Songkla University,Songkhla 90110,Thailand [2]Laboratory Animal Service Center,Faculty of Science,Prince of Songkla University,Songkhla 90110,Thailand [3]Department of Pharmaceutical Chemistry and Pharmacognosy,Faculty of Pharmaceutical Sciences,Naresuan University,Phitsanulok,65000,Thailand

出　　处：《Asian Pacific Journal of Tropical Biomedicine》2023年第5期222-232,共11页亚太热带生物医学杂志（英文版）

基　　金：funded by Prince of Songkla University(Grant No.SCI6302160S)。

摘　　要：Objective:To investigate the in vitro antioxidant and wound healing properties of the hydroethanolic extract of Sargassum polycystum,and elucidate the mechanism of its wound healing activity.Methods:Human dermal fibroblast and HaCaT cells were used to evaluate the proliferation by sulforhodamine B and dsDNA assay after treatment with Sargassum polycystum extracts.Scratch wound healing and phalloidin-rhodamine staining were employed to observe migratory activity and filopodia formation,respectively.Western blot and real-time RT-PCR assays were performed to determine the protein and gene expressions related to wound healing activities.Results:The phytochemical analysis found a higher level of flavonoid than phenolic compound in Sargassum polycystum extracts.In human dermal fibroblast cells,Sargassum polycystum extracts at 50 and 100μg/mL significantly increased fibroblast proliferation and the gene expressions of hyaluronic acid synthase 1(HAS1),HAS2,HAS3,collagen type 1 alpha 1 chain(COL1A1),collagen type 3 alpha 1 chain(COL3A1),and elastin.The phosphorylation of Akt,ERK1/2,and p38 MAPK was also significantly upregulated after treatment with Sargassum polycystum extracts.Additionally,50 and 100μg/mL of the extracts prominently enhanced the proliferation,migration,and filopodia formation of HaCaT cells,as well as the protein levels of pFAK/FAK,pSrc/Src,pAkt/Akt,pERK1/2/ERK1/2,Rac1 and Cdc42.Conclusions:Sargassum polycystum extracts show promising wound healing activities in human dermal fibroblasts and keratinocytes.

关 键 词：Dermal fibroblast Sargassum polycystum Wound healing ANTIOXIDANT PROLIFERATION 

分 类 号：R318[医药卫生—生物医学工程]