益肺灸及其联合调补肺肾三方对COPD稳定期大鼠气道MUC5AC和MUC5B的影响  

Effects of Yifei moxibustion and its combinations with Tiao bu-Fei shen formulas on MUC5AC and MUC5B in the airways of stable COPD rats

作　　者：李景梅 沈婷婷[1,2,4] 范正媛 李亚 李建生 李素云[2,3,4] LI Jingmei;SHEN Tingting;FAN Zhengyuan;LI Ya;LI Jiansheng;LI Suyun(Henan University of Chinese Medicine,Zhengzhou 450046,China;Co-construction Collaborative Innovation Center for Chinese Medicine and Respiratory Diseases by Henan&Education Ministry of P.R.,Zhengzhou 450046;Henan Key Laboratory of Chinese Medicine for Respiratory Disease,Zhengzhou 450046;the Level Three Laboratory of Respiration Pharmacology of TCM in the First Affiliated Hospital of Henan University of Chinese Medicine,Zhengzhou 450000)

机构地区：[1]河南中医药大学,郑州450046 [2]呼吸疾病中医药防治省部共建协同创新中心,郑州450046 [3]河南省中医药防治呼吸病重点实验室,郑州450046 [4]河南中医药大学第一附属医院中药药理(呼吸)实验室,郑州450000

出　　处：《中国比较医学杂志》2023年第4期20-27,共8页Chinese Journal of Comparative Medicine

基　　金：国家重点研发计划项目(2018YFC1704801);国家自然科学基金资助项目(82074413);中医药传承与创新“百千万”人才工程(岐黄工程)项目(国中医药人教函[2018]284号);河南省科技研发计划联合基金项目(222301420081)。

摘　　要：目的探讨益肺灸及其联合调补肺肾三方对慢性阻塞性肺疾病(chronic obstructive pulmonary disease,COPD)稳定期大鼠气道黏蛋白5AC(mucin 5AC,MUC5AC)和黏蛋白5B(mucin 5B,MUC5B)水平的影响,阐明益肺灸作用机制和灸药联合是否具备治疗优势。方法采用香烟烟雾暴露联合肺炎克雷伯杆菌感染法建立COPD稳定期大鼠模型。第9~20周,空白组和模型(COPD)组给予生理盐水(每只2 mL/d)、氨茶碱组给予氨茶碱(27 mg/(kg·d))、补肺健脾组和益肺灸+补肺健脾组给予补肺健脾方(12.42 g/(kg·d))、补肺益肾组和益肺灸+补肺益肾组给予补肺益肾方(11.61 g/(kg·d))、益气滋肾组和益肺灸+益气滋肾组给予益气滋肾方(12.42 g/(kg·d))灌胃,益肺灸组及灸药联合组每周益肺灸灸2次,每次10~15 min。检测大鼠肺功能、肺组织病理、以及支气管肺泡灌洗液(BALF)和气道中MUC5AC、MUC5B蛋白表达。结果COPD组大鼠较空白组Penh水平显著升高(P=0.007),支气管管壁增厚,管腔狭窄,小气道和BALF中MUC5AC蛋白表达均显著升高(P<0.001,P=0.005),MUC5B有升高趋势(P>0.05)。各治疗干预均显著降低COPD大鼠Penh以及BALF和气道MUC5AC水平(P<0.05),益肺灸、调补肺肾三方和益肺灸+益气滋肾方有降低COPD大鼠MUC5B的趋势(P>0.05)。灸药联合在降低Penh、MUC5AC或MUC5B气道表达、分泌方面有不同程度上的优于灸、药单独应用的趋势。结论益肺灸、调补肺肾三方及其联合均可改善COPD大鼠气道通气功能和黏蛋白高表达、分泌,其中,灸药联合可能是更有效策略。Objective To explore the effects of Yifei moxibustion and its combinations with Tiao bu-Fei shen formulas on mucin(MUC)5AC and MUC5B in stable chronic obstructive pulmonary disease(COPD)rats,reveal the mechanism of Yifei moxibustion,and determine whether the combinations are more effective for COPD treatment.Methods Stable COPD rat models were established by cigarette smoke exposure combined with Klebsiella Pneumoniae infection.Rats in control and COPD groups were administrated normal saline(2 mL/d),while those in treatment groups were treated by gavage with the corresponding drugs(APL,27 mg/(kg·d);BJF,12.42 g/(kg·d);BYF,11.61 g/(kg·d);ZSF,12.42 g/(kg·d)or Yifei Moxibustion(twice weekly,10~15 min/time)during week 9~20.Lung functions,pathological changes and expression of MUC5AC and MUC5B in BALF and airways were determined.Results Compared with control rats,COPD rats had a higher level of Penh(P=0.007),thickened bronchial walls,narrowed airways,higher expression of MUC5AC(P<0.001,P=0.005),and higher expression of MUC5B(P>0.05)in small airways and BALF.Compared with the COPD group,Penh and MUC5AC were significantly reduced in all treatment groups(P<0.05).MUC5B in YFM,BJ,BY,ZS,and YFM+ZS groups tended to show decreases(P>0.05).Additionally,combination groups tended to be superior to YFM,BJ,BY,or ZS group alone in reducing the levels of Penh,MUC5AC,or MUC5B to varying degrees.Conclusions Yifei moxibustion,Tiao bu-Fei shen formulas and their combinations improve the airway ventilation function and expression and secretion of mucin in the airways of COPD rats.Additionally,the combinations might be more effective.

关 键 词：慢性阻塞性肺疾病 黏液高分泌 MUC5AC MUC5B 益肺灸 调补肺肾三方 

分 类 号：R-33[医药卫生]