柯萨奇病毒B组1型感染叙利亚金黄地鼠动物模型建立  

Establishment of an animal model of Coxsackievirus B1 infecting Syrian golden hamster

作　　者：李咏洁 张伟 杨凤梅[1] 李艳艳[1] 段素琴[1] 唐洁[1] 孙文亭 靳玮华 邓自君 李明学[1] 张名 马绍辉[1] 和占龙[1] LI Yongjie;ZHANG Wei;YANG Fengmei;LI Yanyan;DUAN Suqin;TANG Jie;SUN Wenting;JIN Weihua;DENG Zijun;LI Mingxue;ZHANG Ming;MA Shaohui;HE Zhanlong(Institute of Medical Biology,Chinese Academy of Medical Sciences/Peking Union Medical College,Kunming 650118,China;the First Affiliated Hospita,Xi’an Jiaotong University,Xi’an 710061)

机构地区：[1]中国医学科学院/北京协和医学院医学生物学研究所,昆明650118 [2]西安交通大学医学部第一附属医院,西安710061

出　　处：《中国比较医学杂志》2023年第4期50-56,共7页Chinese Journal of Comparative Medicine

基　　金：云南省重大科技专项计划(202002AA100009);中国医学科学院医学与健康科技创新工程(2021-I2M-1-024);国家重点研发计划(2021YFF0702804)。

摘　　要：目的建立叙利亚金黄地鼠CVB1(Coxsackievirus B1,CVB1)感染动物模型。方法取叙利亚金黄地鼠经鼻腔滴注方式呼吸道感染CVB1,感染剂量每只为10^(7.25)CCID_(50)。观察14 d体重、体温、精神状态、皮肤黏膜变化、行为、粪便状态、是否有神经症状等临床情况;每天采集咽拭子、鼻灌洗液以及粪便进行病毒载量检测,感染第7天取3只实施安乐死,采血进行病毒载量及生化检测;同时采集脑、心脏、肝等多个组织样品进行病毒载量、组织病理学和IHC检测。结果感染CVB1病毒的动物在14 d内均出现不同程度精神萎靡、体温下降,以及口唇部出现典型的红疹及疱疹等类似人类手足口病临床表现。咽拭子、鼻灌洗液、粪便以及血液中能检测到病毒;组织中检测到病毒载量并观察到病毒抗原,同时伴有炎症、增生、出血等病理改变;血清酶中肝功、心肌酶升高。结论CVB1病毒经滴鼻方式感染叙利亚金黄地鼠建立的感染模型,表现出心肌和肝等组织器官的病理损伤特征,可用于人类手足口病的研究。Objective To establish an animal model of infecting Syrian golden hamsters with CVB1(Coxsackievirus B1,CVB1).Methods Syrian golden hamsters were infected with CVB1 by intranasal instillation at a dose of 10^(7.25) CCID_(50),and were observed clinically for 14 days during which throat swabs,nasal lavage fluid and feces were collected and tested daily for viral load.On the 7th day of infection,3 hamsters were euthanized,and blood samples were collected for viral load and biochemical detection.At the same time,tissue samples were collected for viral load,histopathology and IHC detection.Results The animals infected with CVB1 virus showed varying degrees of listlessness,decreased body temperature,and typical red rash and herpes on the mouth within 14 days,similar to the clinical manifestations of human HFMD.Viruses could be detected in throat swabs,nasal lavage fluid,stool,and blood;viral load was detected and viral antigen was observed in the tissues,accompanied by pathological changes such as inflammation,hyperplasia and hemorrhage;liver functional and myocardial enzymes in serum increased.Conclusions The model of infecting Syrian golden hamsters with CVB1 virus by nasal drip showed pathological damages of tissues and organs such as myocardium and liver,which indicates that the model can be used for the study of human hand-foot-mouth disease.

关 键 词：柯萨奇病毒B组1型 叙利亚金黄地鼠 手足口病 

分 类 号：R-33[医药卫生]